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Recombinant Human Lymphotoxin alpha1/beta2 Protein

Name: Recombinant Human Lymphotoxin alpha1/beta2 Protein
Brand: R&D Systems
SKU: 8884-LY
Price: 590 USD
Availability: InStock

R&D Systems | Catalog # 8884-LY

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Citations (18)

Product Documents

Defined heterotrimer stoichiometry

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Product Specifications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems HEK293-derived Recombinant Human Lymphotoxin alpha1/beta2 Protein (8884-LY)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

P01374 (rhLT alpha ), Q06643 (rhLT beta ) & Q06643 (rhLT beta )

Structure / Form

GS-linked heterotrimer

Applications

Bioactivity

View all Lymphotoxin Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Lymphotoxin protein

rhLT alpha (Leu35-Leu205) Accession # P01374	GGGGS	rhLT beta (Gln49-Gly244) Accession # Q06643	GGGGS	rhLT beta (Gln49-Gly244) Accession # Q06643
N-terminus				C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu35

Predicted Molecular Mass

61 kDa

SDS-PAGE

65-76 kDa, reducing conditions

Activity

Measured by its ability to induce IL-8 secretion by A375 human melanoma cells.
The ED₅₀ for this effect is 3-15 ng/mL.

Formulation, Preparation, and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 8884-LY

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 500 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Carrier Free: 8884-LY/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in sterile PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Lymphotoxin

Lymphotoxin alpha (LT-alpha ), and Lymphotoxin beta (LT-beta ) are pro-inflammatory TNF superfamily ligands that play important roles in immune system development (1, 2). The 25 kDa mature human LT-alpha is a secreted protein that shares 75% amino acid (aa) sequence identity with mouse and rat LT-alpha (3, 4). The 33 kDa mature human LT-beta is a type II transmembrane protein that shares 73% aa sequence identity with mouse and rat LT-beta within common regions of their extracellular domains (5). Relative to the human protein, mouse and rat LT-beta have a 66 aa or 65 aa insertion within the ECD, respectively. LT-alpha can be secreted as a homotrimer that binds and activates TNF RI/TNFRSF1A, TNF RII/TNFRSF1B, HVEM/TNFRSF14, and Troy/TNFRSF19 (6-8). LT-alpha is required for development of the spleen, lymph nodes, and Peyer’s patches (9). It also regulates T cell homing to the gut and IgA induction (10). In addition, LT-alpha can form membrane-associated heterotrimers with two copies of LT-beta on the surface of B, T, LTi, and ILC3 cells (2, 5, 11). The Lymphotoxin alpha 1/ beta 2 heterotrimer binds and activates the Lymphotoxin beta R/TNFRSF3 (LT beta R) which is expressed on macrophages, dendritic cells, hepatocytes, intestinal epithelial cells (IEC), follicular dendritic cells (FDC), and high endothelial venules (HEV) (2, 12, 13). LT beta R also serves as a receptor for LIGHT/TNFSF14 (14). LT-alpha 1/ beta 2 promotes the development of FDC networks and HEV in lymphoid tissue, the class switching of immature B cells for IgA production, and the production of homeostatic IL-22 by ILCs (10, 15-17). It can be shed by ADAM17 or MMP-8 mediated cleavage, and the released heterotrimer circulates in the serum and is elevated in synovial fluid of rheumatoid arthritis patients (18).