Recombinant Human Lymphotoxin alpha2/beta1 Protein

  (2 citations)     
Datasheet / CoA / SDS
Product Details
Citations (2)
FAQs
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  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells. The ED50 for this effect is 0.5‑3 ng/mL.
  • Source
    Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Lymphotoxin protein
    Leu35-Leu205 (LT alpha ) & Leu54-Gly244 (LT beta ), both with an N-terminal Met & Thr41 ‑ Leu205 (LT alpha )
  • Accession #
  • N-terminal Sequence
    Analysis
    Met & Thr41 (LT alpha ) & Met (LT beta )
  • Structure / Form
    Noncovalently-linked heterotrimer
  • Predicted Molecular Mass
    18 kDa (LT alpha ), 18.5 kDa ( LT beta )
  • SDS-PAGE
    18-26 kDa, reducing conditions
Product Datasheets

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Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
679-TX
 
679-TX/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
 
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lymphotoxin

Lymphotoxin-alpha (LT-alpha ), also known as tumor necrosis factor (TNF) beta, and lymphotoxin-beta (LT-beta ) are members of the TNF family. LT-alpha cDNA encodes a 205 amino acid secreted soluble protein with a 34 amino acid signal sequence. LT-beta cDNA encodes a 244 amino acid type II membrane protein with an N-terminal cytoplasmic domain (residues 1 - 18), a transmembrane region (residues 19 - 48), and an extracellular domain (residues 49 - 244). Secreted LT-alpha assembles as a soluble homotrimer, LT-alpha 3. In addition, secreted LT-alpha also complexes with the membrane associated LT-beta to generate two types of heterotrimers, LT-alpha 1/ beta 2 and LT-alpha 2/ beta 1 (1). The soluble LT-alpha 3 binds both TNF RI (p55) and TNF RII (p75). In contrast, the predominant membrane-bound heterotrimer, LT-alpha 1/ beta 2, binds only to the lymphotoxin beta receptor (LT beta R). LT-alpha 2/ beta 1 is capable of binding LT beta R, TNF RI (p55), and TNF RII (p75). LT plays a role in normal lymphoid organogenesis (2, 3). Transgenic LT-alpha knock-out mice exhibited a loss in lymph node development, a change in splenic architecture, and impaired germinal center formation (4). LT is expressed by activated naive CD4 cells, unpolarized IL-2-secreting effectors, and Th1 effectors. A loss of LT expression and lack of TNF-alpha or TNF-beta secretion is associated with prior exposure to IL-4 and a Th2 phenotype (5).

  • References:
    1. Browning et al. (1993) Cell 72:847.
    2. Ettinger et al. (1996) PNAS 93:13102.
    3. Cuff et al. (1998) J. Immunol. 161:6853.
    4. Browning et al. (1997) J. Immunol. 159:3288.
    5. Gramaglia et al. (1999) J. Immunol. 162:1333.
  • Entrez Gene IDs:
    4049 (Human); 16992 (Mouse)
  • Alternate Names:
    LT; Lymphotoxin alpha; Lymphotoxin; tnfb; TNFSF1; TNLG1E
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Chronic lung injury by constitutive expression of activation-induced cytidine deaminase leads to focal mucous cell metaplasia and cancer.
    Authors: Kitamura, Jiro, Uemura, Munehiro, Kurozumi, Mafumi, Sonobe, Makoto, Manabe, Toshiaki, Hiai, Hiroshi, Date, Hiroshi, Kinoshita, Kazuo
    PLoS ONE, 2015;10(2):e0117986.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. LIGHT, a member of the tumor necrosis factor ligand superfamily, prevents tumor necrosis factor-alpha-mediated human primary hepatocyte apoptosis, but not Fas-mediated apoptosis.
    Authors: Hikichi Y, Tsuji I, Shintani Y
    J. Biol. Chem., 2002;277(51):50054-61.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay

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