Recombinant Human LYPD6 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10073-LY-050
Recombinant Human LYPD6 Fc Chimera Protein, CF Binding Activity
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Recombinant Human LYPD6 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human LYPD6 Fc Chimera is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human Frizzled-8 Fc Chimera binds with an ED50 of 2-12 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human LYPD6 protein
Human LYPD6
(Ala23-His152)
Accession # Q86Y78-1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Ala23
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
41 kDa
SDS-PAGE
58-66 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10073-LY

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human LYPD6 Fc Chimera (Catalog # 10073-LY) is coated at 2 μg/mL, 100 μL/well, Biotinylated Recombinant Human Frizzled-8 Fc Chimera binds with an ED50 of 2-12 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human LYPD6 Fc Chimera was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 58-66 kDa and 120 -130 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LYPD6

LYPD6 (Ly6/PLAUR domain-containing protein 6) is a member of the Ly6/uPar family of proteins that are either secreted or GPI-anchored to the cell surface. Members of this protein family include immune regulatory molecules like CD59, the urokinase plasminogen activator receptor (uPar) and molecules that regulate cell proliferation, migration and adhesion (1-3). Ly6/uPar proteins are characterized by the presence of variable loop regions which confer the functional diversity of this protein family (4). Human LYPD6 is synthesized as a 171 aa protein that includes a 22 aa signal peptide and a 149 aa LYPD6 chain. Within the main chain region, human LYPD6 shares 95% sequence identity with mouse LYPD6. LYPD6 is an important regulator of embryogenesis in zebrafish through its enhancement of Wnt/ beta -catenin signaling (5). It is widely expressed in human tissues with higher expression level in the brain and the heart (5-7). Studies have shown LYPD6 can interact with Frizzled-8 and LRP6, and it is required for Wnt-8-mediated mesoderm and neuroectoderm patterning (5). Also, it has been shown LYPD6 can interact with several nicotinic acetylcholine receptors (nAChRs) in the human brain (7). Soluble recombinant LYPD-6 can inhibit nicotine-induced phosphorylation of ERK in PC12 cells and attenuate nicotine-induced hippocampal inward currents in rat brain slices (7).

References
  1. Kong, B.H. and J.H. Park (2012) BMB Rep. 45:595.
  2. Bamezai, A. (2004) Arch. Immunol. Ther. Exp. (Warsz.) 52:255.
  3. Gumley, T.P. et al. (1995) Immunol. Cell. Biol. 73:277.
  4. Vasilyeva, N.A. et al. (2017) Biochemistry (Mosc.) 82:1702.
  5. Özhan, G. et al. (2013) Dev Cell. 26(4):331.
  6. Zhang, Y. et al. (2010) Mol Biol Rep 37:2055.
  7. Arvaniti, M. et al. (2016) J. Neurochem. 138:806.
Long Name
Ly6/PLAUR Domain-containing Protein 6
Entrez Gene IDs
130574 (Human); 320343 (Mouse); 679564 (Rat)
Alternate Names
LY6/PLAUR domain containing 6; LYPD6; UNQ3023/PRO9821

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