Recombinant Human Matrilin-2 Protein, CF

  (1 citations)     
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Citations (1)
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  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized rat tail Collagen I at 10 µg/mL (100 µL/well) can bind rhMatrilin-2 with a linear range of 16-1,000 ng/mL.
  • Source
    Mouse myeloma cell line, NS0-derived human Matrilin-2 protein Arg24-Arg937, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Arg24
  • Predicted Molecular Mass
    103 kDa
  • SDS-PAGE
    120 kDa, reducing conditions
Product Datasheets

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Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3044-MN/CF
 
3044-MN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
 
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Background: Matrilin-2
Matrilin-2 is an extracellular matrix protein that belongs to the superfamily of von Willebrand factor A (VWA) containing proteins. It is expressed in many tissues and functions as a bridging component between other matrix proteins (1 - 4). The human Matrilin-2 cDNA encodes a 956 amino acid (aa) precursor with a 23 aa signal sequence, two VWA domains separated by ten tandem EGF-like repeats, and a C-terminal coiled-coil domain (5, 6). Alternate splicing generates Isoform 2 (with an 18 aa deletion near the C-terminus), Isoform 3 (with a deletion of the fourth EGF-like repeat), and Isoform 4 (with a deletion of the first VWA and first EGF-like repeat). Human Matrilin-2 shares 87% and 84% aa sequence identity with mouse and canine Matrilin-2, respectively, and 27%, 22%, and 33% aa sequence identity with human Matrilin-1, -3, and -4, respectively. Matrilin-2 forms a variety of disulfide-linked oligomers via its coiled-coil domain (4, 7, 8, 9). It can assemble into homotrimers or heterotrimers with Matrilin-1 and/or Matrilin-4 (4, 7, 8) but has not been detected in heterotrimers containing Matrilin-3 (8). The VWA domains are thought to mediate Matrilin-Matrilin interactions as well as interactions with other matrix proteins such as Fibronectin, Collagen I, Fibrillin-2, and Laminin-1/Nidogen-1 complexes (7). Matrilin-2 knockout mice do not display any obvious abnormalities, suggesting that the expression of other molecules can compensate for the lack of Matrilin-2 (10).
  • References:
    1. Wagener, R. et al. (2005) FEBS Lett. 579:3323.
    2. Deak, F. et al. (1999) Matrix Biol. 18:55.
    3. Whittaker, C.A. and R.O. Hynes (2002) Mol. Biol. Cell 13:3369.
    4. Piecha, D. et al. (1999) J. Biol. Chem. 274:13353.
    5. Muratoglu, S. et al. (2000) Cytogenet. Cell Genet. 90:323.
    6. Deak, F. et al. (1997) J. Biol. Chem. 272:9268.
    7. Piecha, D. et al. (2002) Biochem. J. 367:715.
    8. Frank, S. et al. (2002) J. Biol. Chem. 277:19071.
    9. Pan, O.H. and K. Beck (1998) J. Biol. Chem. 273:14205.
    10. Mates, L. et al. (2004) Matrix Biol. 23:195.
  • Entrez Gene IDs:
    4147 (Human)
  • Alternate Names:
    MATN2; matrilin 2; Matrilin2; Matrilin-2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. ADAMTS5 Deficiency in Calcified Aortic Valves Is Associated With Elevated Pro-Osteogenic Activity in Valvular Interstitial Cells
    Authors: F Li, R Song, L Ao, TB Reece, JC Cleveland, N Dong, DA Fullerton, X Meng
    Arterioscler. Thromb. Vasc. Biol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay

FAQs

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Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB , Simple Western , Flow , AP , CyTOF-ready MAB050 31  
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His Tag HRP-conjugated Antibody

WB , Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 3  
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His Tag Antibody

WB , Flow , AP MAB050R 2  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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