Recombinant Human mGluR4 Protein, CF

R&D Systems | Catalog # 9528-UR

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human mGluR4 Protein (9528-UR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q14833-1

Structure / Form

Disulfide-linked homodimer
View all mGluR4 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human mGluR4 protein
Lys33-Ser518, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys33

Predicted Molecular Mass

55 kDa

SDS-PAGE

62-76 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation, and Storage

9528-UR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: mGluR4

Metabotropic glutamate receptors (mGluRs) are members of the G-protein-coupled receptor (GPCR) superfamily, modulating glutamate neurotransmission in the central and peripheral nervous systems through GTP-binding proteins (1). Structurally, members of this family are characterized by a large N-terminal extracellular domain (ECD), seven transmembrane domain, and a cytoplasmic C-terminal domain that is variable in length. Two ECDs dimerize together and large conformational changes are induced when agonists bind to one or both domains (2). The C-terminal region is subject to alternative splicing, regulation by phosphorylation and interacts directly with a G-protein to modulate protein-protein interactions (2, 3). The receptors are subdivided into three groups (I–III) based on sequence homology, signal transduction and pharmacological properties (1, 2). The Group III receptors include mGLuRs 4, 6, 7 and 8 (2). mGLuR4 has been shown to be expressed postsynaptically in certain areas such as the hippocampus and retina (4). Mature human mGluR4 is 880 amino acids in length. In the ECD region comprising amino acids (aa) 33-587, human mGluR4 shares 97% aa sequence identity with mouse and rat mGluR4.

References

  1. Conn P.J. et al. (1997) Annu. Rev. Pharmacol. Toxicol. 37:205.
  2. Niswender C.M. et al. (2010) Annu. Rev. Pharmacol. Toxicol. 5:295.
  3. Pin J.P. et al. (1995) Neuropharmacology. 34:1.
  4. Bradley, S.R. et al. (1996) J. Neurosci. 16:2044.

Long Name

Metabotropic Glutamate Receptor 4

Alternate Names

GPRC1D, GRM4

Entrez Gene IDs

2914 (Human); 268934 (Mouse); 24417 (Rat)

Gene Symbol

GRM4

UniProt

Q14833-1

Additional mGluR4 Products

Product Documents for Recombinant Human mGluR4 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human mGluR4 Protein, CF

For research use only

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