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Recombinant Human/Mouse Indian Hedgehog/Ihh (C28II) N-Term

Catalog #: 1705-HH
8 Citations Datasheet / COA / SDS
Newer Version Available: 1705-HH/CF
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Discontinued Product

1705-HH has been discontinued and is replaced by 1705-HH/CF.

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Recombinant Human/Mouse Indian Hedgehog/Ihh (C28II) N-Term Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is 3-12 µg/mL.
Source
E. coli-derived Indian Hedgehog/Ihh protein
Cys28-Gly202 (Cys28Ile-Ile), with an N-terminal Met
Accession #
P97812.2
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
20 kDa

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1705-HH (with carrier)

Product Datasheet
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1705-HH/CF (carrier free)

Discontinued Product

1705-HH

Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and DTT with Trehalose and with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile water containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Indian Hedgehog/Ihh

The hedgehog (hh) gene encoding a secreted protein was originally identified in Drosophila as a segment polarity gene. The vertebrate homologues of Hh comprise several proteins including sonic hedgehog (Shh), Indian hedgehog (Ihh), and Desert hedgehog (Dhh) (1). Hedgehog proteins are important signaling molecules during embryonic development and are highly conserved within and across species (1). Mouse and human Ihh share 100% amino acid identity in the signaling domain, while mouse Ihh and Shh share 90% amino acid identity in the N-terminal signaling domain. Ihh mRNA expression is detected in fetal lung, gut, stomach, liver, kidney, pancreas and strongly in cartilage - in growth regions of the developing bone (2, 3). Ihh, along with parathyroid hormone related protein, regulate the rate of chondrocyte proliferation and differentiation (4). Ihh is also involved in yolk sac vasculogenesis, playing an important role in differentiation of epiblast cells into endothelial and red blood cells (5).

Mouse Ihh cDNA encodes a 411 amino acid (aa) polypeptide with a predicted 27 aa signal peptide. This polypeptide is cleaved to generate a 45 kDa precursor protein that undergoes the same post‑translation processing as Shh (3). An autocatalytic reaction yields a 19 kDa amino‑terminal domain Ihh‑N protein that retains all known signaling capabilities, and a 23 kDa carboxy‑terminal domain Ihh-C protein (3). Since hydrophobic modifications to Shh, including the substitution of the N-terminal cysteine residue with two hydrophobic isoleucine residues, can also increase its potency (6), a similar modification was made for Ihh. This modified form also shows increased potency in a bioassay measuring induction of alkaline phosphatase. At the cell surface, Hedgehog activity is mediated by a multicomponent receptor complex involving the 12‑pass transmembrane protein Patched (Ptc) which binds Hedgehogs with high affinity and Smoothened (Smo), a signaling seven transmembrane G-protein coupled receptor (1).

References
  1. Ingham, P. and A. McMahon (2001) Genes & Dev. 15:3059.
  2. Marigo, V. et al. (1995) Genomics 28:44.
  3. Valentini, R.P. et al. (1997) J Biol Chem. 272:8466.
  4. Vortkamp, A. et al. (1996) Science 273:613.
  5. Byrd, N. et al. (2002) Development 129:361.
  6. Taylor, F.R. et al. (2001) Biochemistry 40:4359.
Entrez Gene IDs
3549 (Human); 16147 (Mouse)
Alternate Names
BDA1; BDA1Indian hedgehog homolog (Drosophila); HHG2; HHG-2; Ihh; Indian hedgehog (Drosophila) homolog; Indian hedgehog homolog; indian hedgehog protein; Indian Hedgehog

Citations for Recombinant Human/Mouse Indian Hedgehog/Ihh (C28II) N-Term

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Altered IHH signaling contributes to reduced chondrocyte proliferation in the growth plate of MPS VII mice
    Authors: Z Jiang, ALK Derrick-Ro, S Byers
    Mol Genet Metab Rep, 2020-10-22;25(0):100668.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Regulation of Calvarial Osteogenesis by Concomitant De-repression of GLI3 and Activation of IHH Targets
    Authors: LK Veistinen, T Mustonen, MR Hasan, M Takatalo, Y Kobayashi, DA Kesper, A Vortkamp, DP Rice
    Front Physiol, 2017-12-19;8(0):1036.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Endogenously produced Indian Hedgehog regulates TGFbeta-driven chondrogenesis of human bone marrow stromal/stem cells.
    Authors: Handorf A, Chamberlain C, Li W
    Stem Cells Dev, 2015-01-26;24(8):995-1007.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. The osteoblast to osteocyte transition: epigenetic changes and response to the vitamin D3 hormone.
    Authors: St John, Hillary, Bishop, Kathleen, Meyer, Mark B, Benkusky, Nancy A, Leng, Ning, Kendziorski, Christin, Bonewald, Lynda F, Pike, J Wesley
    Mol Endocrinol, 2014-05-30;28(7):1150-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Chondrocyte proliferation regulated by secreted luminal domain of ER stress transducer BBF2H7/CREB3L2.
    Authors: Saito A, Kanemoto S, Zhang Y, Asada R, Hino K, Imaizumi K
    Mol Cell, 2013-12-12;53(1):127-39.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  6. Plasma Shh Levels Reduced in Pancreatic Cancer Patients
    Authors: Mohamad El-Zaatari, Stephanie Daignault, Art Tessier, Gail Kelsey, Lisa A. Travnikar, Esperanza F. Cantu et al.
    Pancreas
    Species: Human
    Sample Types: Plasma, Whole Cells
    Applications: Bioassay, ELISA Standard
  7. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Signaling hierarchy regulating human endothelial cell development.
    Authors: Kelly MA, Hirschi KK
    Arterioscler. Thromb. Vasc. Biol., 2009-02-12;29(5):718-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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