Growth Differentiation Factor 8 (GDF-8), also known as myostatin, is a member of the TGF-beta superfamily that is expressed specifically in developing and adult skeletal muscle. GDF-8 cDNA encodes a 376 amino acid (aa) prepropeptide with a 24 aa residue signal peptide, a 223 aa residue amino-terminal propeptide, and a 109 aa residue carboxy-terminal mature protein. Mature GDF-8 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members. Similar to the TGF‑ beta s, activins and BMP-11, GDF-8 also contains one extra pair of cysteine residues that is not found in other family members. The bioactive form of GDF-8 is a homodimer with an apparent molecular weight of approximately 25 kDa. GDF-8 is highly conserved across species. At the amino acid sequence level, mature human, mouse, rat and cow GDF-8 are 100% identical. Within the TGF-beta superfamily, GDF-8 is most closely related to BMP-11, a mammalian protein that acts as a dorsal mesoderm and neural inducer in Xenopus explants. The two proteins share 90% amino acid sequence identity within their mature chain. A targeted disruption of GDF-8 in mouse results in large mice with a widespread increase in skeletal muscle mass, indicating that GDF-8 is a negative regulator of skeletal muscle growth. A mutation in the bovine GDF-8 gene has been shown to be responsible for the double-muscled phenotype in cattle breeds such as Belgian Blue cattle that is characterized by an increase in muscle mass. GDF-8 has also been shown to inhibit preadipocyte differentiation to adipocytes. Mature GDF-8 binds to activin type II receptors and the binding is antagonized by the activin-binding protein, follistatin. R&D Systems recombinant GDF-8 preparations have been shown to act similarly to Activin A in both the Xenopus animal cap and the K562 assays.
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Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein
R&D Systems | Catalog # 788-G8
GMP Version Available:
788-GMP
GMP
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Key Product Details
- R&D Systems NS0-derived Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein (788-G8)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Structure / Form
Disulfide-linked homodimer
Applications
Bioactivity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived GDF-8/Myostatin protein
Asp268-Ser376
Asp268-Ser376
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Asp268
Predicted Molecular Mass
12.4 kDa
SDS-PAGE
12 kDa, reducing conditions
24 kDa, non-reducing conditions
24 kDa, non-reducing conditions
Activity
Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340.
The ED50 for this effect is 2-10 ng/mL.
The ED50 for this effect is 2-10 ng/mL.
Reviewed Applications
Read 3 reviews rated 4.3 using 788-G8 in the following applications:
Scientific Data Images for Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein
Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein Bioactivity
Recombinant Human/Mouse/Rat GDF-8/ Myostatin (Catalog # 788-G8) induces hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The ED50 for this effect is 2-10 ng/mL.
Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein SDS-PAGE
1 μg/lane of Recombinant Human/Mouse/Rat GDF-8/Myostatin was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing the main bands at 12 kDa and 24 kDa, respectively.Formulation, Preparation, and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 788-G8
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 788-G8/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 4 mM HCl. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: GDF-8/Myostatin
References
- Storm, E.E. et al. (1994) Nature 368:639.
- Sharma, M. et al. (1999) J. Cell Physiol. 180:1.
- McPherron, A.C. et al. (1997) Nature 387:83.
- Lee, S.J. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9306.
- Kim, H.S. et al. (2001) Biochem. Biophys. Res. Commun. 281:902.
Long Name
Growth Differentiation Factor 8
Alternate Names
GDF8, MSLHP, MSTN, Myostatin
Gene Symbol
MSTN
UniProt
Additional GDF-8/Myostatin Products
Product Documents for Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein
Product Specific Notices for Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein
For research use only
Related Research Areas
Citations for Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein
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Customer Reviews for Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein (3)
4.3 out of 5
3 Customer Ratings
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Application: Immunoassay StandardVerified Customer | Posted 04/15/2024Upon receipt of the recombinant myostatin, i reconstituted it in 4 mM HCl. I had loaded 1μg of recombinant myostatin/GDF8 into a 4-15% gel, performed electrophoresis with MES buffer and blotted the gel onto 0.2μm nitrocellulose membrane. For the immuno-detection, I had used the R&D systems goat polyclonal GDF8 antibody (BAF788) at 0.1 or 0.25 µg/mL concentration, and the R&D system anti-goat secondary antibody (HAF017) at 1:4000 dilution.
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 04/07/2020
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Application: Immunoassay StandardVerified Customer | Posted 10/20/2017
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FAQs for Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein
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Q: Is Recombinant Human/Mouse/Rat GDF-8/Myostatin Protein (Catalog # 788-G8) the mature form of GDF8?
A: The mature form of GDF8 consists of a disulfide-linked homodimer. Catalog 788-G8 consists of Asp268-Ser376. There are multiple disulfide bonds within this sequence, which form a disulfide-linked heterodimer with another Asp268-Ser376 molecule.