Recombinant Human MSPR/Ron Protein, CF

Catalog # Availability Size / Price Qty
1947-MS-050
Product Details
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Recombinant Human MSPR/Ron Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human MSP R/Ron is immobilized at 100 ng/mL (100 μL/well), the concentration of recombinant human MSP/MST1 that produces 50% optimal binding response is found to be 8-40 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human MSP R/Ron protein
Glu25-Leu571 (single chain) & Gly310-Leu571 (beta), with a C-terminal 10-His tag; Glu25-Arg309 (alpha)
Accession #
N-terminal Sequence
Analysis
Glu25 & Gly310
Structure / Form
Disulfide-linked heterodimer
Predicted Molecular Mass
60 kDa (single chain), 29.7 kDa ( alpha subunit), 29.6 kDa ( beta subunit)
SDS-PAGE
75 kDa, 40 kDa and 38 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1947-MS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: MSPR/Ron

Macrophage stimulating protein receptor (MSP R), encoded by the human Ron and the mouse Stk, is one of a small family of receptor tyrosine kinases (RTKs) that also includes human Met (the receptor for hepatocyte growth factor) and chicken Sea (1, 2). This family of receptors is synthesized as a single-chain precursors that are cleaved into a mature disulfide-linked heterodimers composed of an extracellular alpha chain and a membrane spanning beta chain with intrinsic tyrosine kinase activity. Biologically active ligands (MSP and HGF) for this family of receptors are also disulfide-linked alpha -beta heterodimers. Human MSP R cDNA encodes a 1400 amino acid (aa) residue precursor protein with a 24 aa signal peptide, a 285 aa residue alpha chain (Glu25-Arg309) and a 1091 aa residue transmembrane beta chain (Gly310-Thr1400). The extracellular domain of MSP R is comprised of an N-terminal sema domain, a PSI (plexin semaphorins integrins) domain, followed by four immunoglobulin-like folds shared by plexins and transcription factors (3). The soluble sema domain binds MSP and inhibits the MSP R-dependent signaling pathways. MSP receptor is expressed in multiple tissues including specific areas of the central and peripheral nervous systems, epithelial cells along the digestive tract, skin and lung, and in subpopulations of the mononuclear phagocyte lineage (1, 2). Although free MSP alpha or beta chains have been shown to bind MSP R, only the heterodimeric MSP can induce receptor activation and cause biological activity (4, 5). MSP R associates with other transmembrane molecules including integrins, cadherins and other cytokine receptors. Transactivation and signaling crosstalk between MSP R and its associated transmembrane receptors have been demonstrated (6-8). Human MSP R shares 72% amino acid sequence identity with mouse MSP R.

References
  1. Gaudino, G. et al. (1994) EMBO J. 13:3524.
  2. Wang, M-H. et al. (1994) Science 266:117.
  3. Angelonis, D. et al. (2004) J. Biol. Chem. 279:3726.
  4. Wang, M-H. et al. (1997) J. Biol. Chem. 272:16999.
  5. Danilkovitch, A. et al. (1999) J. Biol. Chem. 274:29937.
  6. Danilkovitch-Miagkova, A. et al. (2000) J. Biol. Chem. 275:14783.
  7. Danilkovitch-Miagkova, A. and A. Leonard (2001) Histol. Histopathol. 16:623.
  8. Santora, M. et al. (2003) Devel. Cell 2:257.
Long Name
Macrophage Stimulating Protein Receptor
Entrez Gene IDs
4486 (Human); 19882 (Mouse)
Alternate Names
CD136 antigen; CD136; CDw136c-met-related tyrosine kinase; EC 2.7.10; EC 2.7.10.1; macrophage stimulating 1 receptor (c-met-related tyrosine kinase); MSP R; MSP receptor; MSPR; MST1R; p185-Ron; Protein-tyrosine kinase 8; PTK8 protein tyrosine kinase 8; PTK8; Ron; RONmacrophage-stimulating protein receptor; soluble RON variant 1; soluble RON variant 2; soluble RON variant 3; soluble RON variant 4

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Reviews for Recombinant Human MSPR/Ron Protein, CF

Average Rating: 4 (Based on 1 Review)

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Recombinant Human MSP R/Ron Protein, CF
By Anonymous on 10/24/2017
Application: Binding assay/Protein-protein interaction
Reason for Rating: The material was shown to be >80% monomer by SEC-MALS.