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Recombinant Human Myc-Ubiquitin Protein, CF

Catalog #: U-115
6 Citations Datasheet / COA / SDS

Discontinued Product

U-115 has been discontinued.
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Citations (6)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Myc-Ubiquitin Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human myc-Ubiquitin can be conjugated to substrate proteins via the subsequent actions of a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human myc-Ubiquitin concentration of 0.5-1 mM.
Source
E. coli-derived human Ubiquitin protein
Contains an N-terminal Myc (EQKLISEEDL) tag
Accession #
P62988
Predicted Molecular Mass
9.9 kDa

Product Datasheets

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U-115

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

U-115

Formulation Lyophilized from a solution in deionized water.
Reconstitution Reconstitute at 10 mg/mL in an aqueous solution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ubiquitin

Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1) (2,3). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting (4-7).

This fully functional N-terminal Myc-tagged Ubiquitin protein allows for the convenient detection or affinity purification of ubiquitinated proteins in vitro. The tag sequence (EQKLISEEDL) is an epitope derived from the C-terminal region of the human proto-oncoprotein p62c-myc. This tag is specifically recognized by Anti-Myc antibodies or Anti-Myc-agarose.

References
  1. Sharp, P.M. & W.-H. Li. (1987) Trends Ecol. Evol. 2:328.
  2. Ciechanover, A. et al. (1980 ) Proc. Natl. Acad. Sci. USA 77:1365.
  3. Hershko, A. et al. (1980) Proc. Natl. Acad. Sci. USA 77:1783.
  4. Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
  5. Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
  6. Wei, W. et al. (2004) Nature 428:194.
  7. Wertz, I.E. et al. (2004) Nature 430:694.
Entrez Gene IDs
7314 (Human); 298693 (Rat)
Alternate Names
RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin

Citations for Recombinant Human Myc-Ubiquitin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Small-molecule inhibitors targeting Polycomb repressive complex 1 RING domain
    Authors: S Shukla, W Ying, F Gray, Y Yao, ML Simes, Q Zhao, H Miao, HJ Cho, P González-A, A Winkler, G Lund, T Purohit, E Kim, X Zhang, JM Ray, S He, C Nikolaidis, J Ndoj, J Wang, ? Jaremko, M Jaremko, RJH Ryan, ML Guzman, J Grembecka, T Cierpicki
    Nature Chemical Biology, 2021-06-21;17(7):784-793.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ubiquitination
  2. CRL4AMBRA1 is a master regulator of D-type cyclins
    Authors: D Simoneschi, G Rona, N Zhou, YT Jeong, S Jiang, G Milletti, AA Arbini, A O'Sullivan, AA Wang, S Nithikasem, S Keegan, Y Siu, V Cianfanell, E Maiani, F Nazio, F Cecconi, F Boccalatte, D Fenyö, DR Jones, L Busino, M Pagano
    Nature, 2021-04-14;592(7856):789-793.
    Species: Human
    Sample Types: Peptide
    Applications: Bioassay
  3. In vitro analysis of RQC activities provides insights into the mechanism and function of CAT tailing
    Authors: BA Osuna, CJ Howard, S Kc, A Frost, DE Weinberg
    Elife, 2017-07-18;6(0):.
    Species: Yeast - Saccharomyces cerevisiae
    Sample Types: RNA
    Applications: Bioassay
  4. Deltex2 represses MyoD expression and inhibits myogenic differentiation by acting as a negative regulator of Jmjd1c
    Authors: D Luo, A de Morree, S Boutet, N Quach, V Natu, A Rustagi, TA Rando
    Proc. Natl. Acad. Sci. U.S.A., 2017-03-28;114(15):E3071-E3080.
    Species: Mouse
    Sample Types: Protein
    Applications: Bioassay
  5. BBX21, an Arabidopsis B-box protein, directly activates HY5 and is targeted by COP1 for 26S proteasome-mediated degradation
    Proc Natl Acad Sci USA, 2016-06-20;113(27):7655-60.
    Species: Plant - Arabidopsis
    Sample Types: Protein, Recombinant Protein
    Applications: Bioassay
  6. A cell cycle-dependent BRCA1-UHRF1 cascade regulates DNA double-strand break repair pathway choice
    Authors: H Zhang, H Liu, Y Chen, X Yang, P Wang, T Liu, M Deng, B Qin, C Correia, S Lee, J Kim, M Sparks, AA Nair, DL Evans, KR Kalari, P Zhang, L Wang, Z You, SH Kaufmann, Z Lou, H Pei
    Nat Commun, 2016-01-05;7(0):10201.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay

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