Recombinant Human Myocilin His-tag Protein, CF Summary
Leu215-Met504, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Recombinant Human Myocilin supporst the adhesion of AGS human Gastric Adenocarcinoma cells. The ED50 for this effect is 0.3-3 μg/mL.
2 μg/lane of Recombinant Human Myocilin His-tag (Catalog# 3446-MY) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 28-35 kDa.
Myocilin is a secreted glycoprotein that belongs to the family of olfactomedin-related proteins (1). Mature human Myocilin is synthesized as a 472 amino acid (aa) precursor that can be cleaved into a 194 aa N-terminal fragment containing leucine zipper motifs within two coil–coil domains and a 335 aa C-terminal fragment containing an olfactomedin (OLF) domain (2). The human OLF-domain shares 87% aa sequence identity with the OLF-domain in mouse and rat. The Myocilin gene is expressed in the human eye compartments. When it is secreted, it can be detected in the cornea, trabecular meshwork, aqueous humor, iris, ciliary body, choroid sclera, retina and the axons of optic nerve ganglion cells (3). Mutations of the MYOC gene are associated with primary open angle glaucoma (POAG), which is a complex disorder with a major heritable component (4). Myocilin interacts with itself to form dimers or multimers (2, 5-8), flotilin-1, optimedin, fibronectin, and fibrilin-1 as well as hevin and SPARC (2, 8-10). Myocilin stimulates cell migration that involves the activation of intergrin focal adhesion kinase (FAK)-serin/threonine kinase (AKT) signaling pathway (11). Myocilin binds specifically to the Heparin II domain of fibronectin (12).
- Zeng, L.C. et al. (2005) FEBS Lett. 579:5443.
- Resch, Z.T. and Fautsch M.P. (2009) Exp Eye Res. 88:704.
- Karali, A. et al. (2000) Invest. Ophthalmol. Vis. Sci. 41:729.
- Ikezoe, T. et al. (2003) Int J Mol Med. 12:259.
- Aroca-Aguilar, J.D. et al. (2013) PLoS One 8:e54385.
- Tamm, E.R. (2002) Prog Retin Eye Res 21:395.
- Fautsch, M.P. et al. (2006) Exp Eye Res 82:1046.
- Aroca-Aguilar, J.D. et al. (2011) Invest Ophthalmol Vis Sci 52:179.
- Wentz-Hunter, K. et al. (2002) Invest Ophthalmol Vis Sci 43:176.
- Ueda, J. et al. (2000) J Histochem Cytochem 48:1321.
- Kwon, H.S. and Tomarev, S.I. (2011) J Cell Physiol 226:3392.
- Filla, M.S. et al. (2002) Invest Ophthalmol Visual Sci. 43:151.
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