Recombinant Human Neprilysin-2/MMEL1 Protein, CF

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Product Details
Citations (1)
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Recombinant Human Neprilysin-2/MMEL1 Protein, CF Summary

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005). The specific activity is >250 pmol/min/µg, as measured under the described conditions.
Mouse myeloma cell line, NS0-derived human Neprilysin-2/MMEL1 protein
Gly69-Trp770, with an N-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
82 kDa
99 kDa, reducing conditions

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Background: Neprilysin-2/MMEL1

Neprilysin-2 is a zinc protease of the neprilysin (NEP) family, which also includes NEP, ECE-2, PEX, DINE, Kell and several NEP-like proteins (1). It is encoded by the MMEL1 gene in the human genome and is also known as soluble secreted endopeptidase (SEP), Neprilysin-like 1 and Neprilysin-2 (2). Highly expressed in testis, the cDNA predicted a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Both membrane-bound and soluble forms were comparable with regard to model substrates, pH optima and inhibitor profiles. The ectodomain of human Neprilysin-2/MMEL1 was expressed with an N-terminal His tag and purified.

  1. Turner, A.J. et al. (2001) BioEssays 23:261.
  2. Carpentier, M. et al. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. eds. p. 426.
Entrez Gene IDs
79258 (Human)
Alternate Names
EC 3.4.24; EC; MELL1; membrane metallo-endopeptidase-like 1; Membrane metallo-endopeptidase-like 2MGC119455; MGC119454; MMEL1; MMEL2; NEP2; NEP2(m); NEPIIMGC119456; Neprilysin II; Neprilysin2; Neprilysin-2; NL2; NL2NL1; SEP; soluble secreted endopeptidase; zinc metallopeptidase

Citation for Recombinant Human Neprilysin-2/MMEL1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Human membrane metallo-endopeptidase-like protein degrades both beta-amyloid 42 and beta-amyloid 40.
    Authors: Huang JY, Bruno AM, Patel CA, Huynh AM, Philibert KD, Glucksman MJ, Marr RA
    Neuroscience, 2008;155(1):258-62.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: EnzAct


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