Recombinant Human Netrin-4 Protein, CF

(5 citations)   
  • Purity
    >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized rrUNC5H2/Fc Chimera at 5 µg/mL (100 µL/well) can bind rhNetrin-4 with a linear range of 6-400 ng/mL.
  • Source
    Mouse myeloma cell line, NS0-derived Val26-Lys628, with a C-terminal 10-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Val26
  • Predicted Molecular Mass
    69 kDa
  • SDS-PAGE
    90 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1254-N4/CF
 
1254-N4
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Netrin-4

Netrins/UNC-6 (netr: Sanskrit for “one who guides”) are a family of laminin-related small proteins that are involved in neurite outgrowth and axon guidance. Netrins bind to the DCC and UNC5 family of receptors to attract or repel axons. Human Netrin-4 is synthesized as a 628 amino acid (aa) precursor that contains a 19 aa signal sequence, a 443 aa laminin-related region containing an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, and a 166 aa C-terminal domain rich in basic aa residues that serves as a heparin binding site. Unlike human Netrin-1 which is reminiscent of the laminin gamma -chain, human Netrin-4 resembles the laminin beta -chain. Netrin-4 has been reported to exist as both a monomer and a dimer. The dimeric form was reported to be the less active of the two. Human Netrin-4 shares 31%, 29% and 25% aa sequence identity with human Netrin-1, 2 and G2, respectively; it shares 89% aa identity with mouse Netrin-4. Netrin-4 has widespread expression, occurring in nervous tissues such as embryonic floor plate and postnatal neurons such as cerebellar granule cells and hippocampal pyramidal cells. It also is found in non-neural tissues, such as adult Bowman’s capsule and medullary tubular epithelium in kidney, and embryonic pancreatic and intestinal epithelium, plus cells of the ureteric bud. Netrin-4 expression is often associated with basement membrane. Netrin-4 has been shown to initiate axon outgrowth from olfactory bulb explants (1 - 7).

  • References:
    1. McFarlane, S. (2000) Biochem. Cell. Biol. 78:563.
    2. Kennedy, T.E. (2000) Biochem. Cell Biol. 78:569.
    3. Yu, T.W. and C.I. Bargmann (2001) Nature Neurosci. 4(Suppl):1169.
    4. Stein, E. et al. (2001) Science 291:1976.
    5. Koch, M. et al. (2000) J. Cell Biol. 151:221.
    6. Yin, Y. et al. (2000) Mech. Dev. 96:115.
    7. Livesey, F.J. (1999) Cell. Mol. Life Sci. 56:62.
  • Entrez Gene IDs:
    59277 (Human); 57764 (Mouse)
  • Alternate Names:
    beta-Netrin; hepar-derived netrin-like protein; netrin 4; Netrin4; Netrin-4; NTN4; PRO3091
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. The Netrin-4/ Neogenin-1 axis promotes neuroblastoma cell survival and migration
    Authors: AA Villanueva, P Falcón, N Espinoza, LS R, LA Milla, E Hernandez-, VA Torres, P Sanchez-Go, V Palma
    Oncotarget, 2017;8(6):9767-9782.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Netrin-1 promotes medulloblastoma cell invasiveness and angiogenesis, and demonstrates elevated expression in tumor tissue and urine of patients with pediatric medulloblastoma.
    Authors: Akino T, Han X, Nakayama H, McNeish B, Zurakowski D, Mammoto A, Klagsbrun M, Smith E
    Cancer Res, 2014;74(14):3716-26.
    Species: N/A
    Sample Type: N/A
    Application: WB Standard
  3. NETRIN-4 protects glioblastoma cells FROM temozolomide induced senescence.
    Authors: Li L, Hu Y, Ylivinkka I, Li H, Chen P, Keski-Oja J, Hyytiainen M
    PLoS ONE, 2013;8(11):e80363.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme.
    Authors: Sneddon, Julie B, Borowiak, Malgorza, Melton, Douglas
    Nature, 2012;491(7426):765-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Cell Culture Substrate
  5. Netrin-4 inhibits angiogenesis via binding to neogenin and recruitment of Unc5B.
    Authors: Lejmi E, Leconte L, Pedron-Mazoyer S, Ropert S, Raoul W, Lavalette S, Bouras I, Feron JG, Maitre-Boube M, Assayag F, Feumi C, Alemany M, Jie TX, Merkulova T, Poupon MF, Ruchoux MM, Tobelem G, Sennlaub F, Plouet J
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(34):12491-6.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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