Recombinant Human Neudesin Protein, CF

Catalog # Availability Size / Price Qty
6714-ND-050
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Recombinant Human Neudesin Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using Neuro‑2A mouse neuroblastoma cells. The ED50 for this effect is 1.5-6.0 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Neudesin protein
Gly32-Phe172, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Starts at Gly32
Structure / Form
Monomer
Predicted Molecular Mass
16.5 kDa
SDS-PAGE
19-21 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6714-ND

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: Neudesin

Neudesin (neuron‑derived neurotrophic secreted protein), gene name NENF (neudesin neurotrophic factor), also called CIR2 (cell immortalization‑related 2) or GIG47, is a secreted, 20‑21 kDa member of the MAPR (membrane‑associated progesterone receptor) subfamily of the cytochrome b5 family of molecules (1, 2). Human Neudesin is synthesized as a 172 amino acid (aa) precursor that contains a 31 aa signal sequence with a 141 aa mature region that possesses a cytochrome b5‑like heme‑binding domain over aa 44‑129, and a lysine acetylation site at aa 136 (1‑4). The attachment of hemin to its heme‑binding domain is necessary for its neurotrophic activity, and the binding of heme accounts for 5‑6 kDa of its circulating molecular weight (3). Mature human Neudesin shares 97%, 94% and 96% aa identity with mouse, rat, and bovine Neudesin, respectively. Neudesin appears to selectively promote cell survival or proliferation and inhibit differentiation in multiple settings. It is expressed by neuronal progenitors and neurons in the central nervous system, and by preadipocytes in white adipose tissue (5, 6). It promotes neuronal differentiation with limited proliferation and serves as a neuron survival factor, but inhibits both astrocyte and adipocyte differentiation (1, 5, 6). Neudesin over‑expression is found in tumors in the human breast, cervix, colon, lung and skin, and in human tumor cell lines that include some lymphomas and leukemias; transfection studies indicate that it may act as an oncogene (4, 7).

References
  1. Kimura, I. et al. (2005) J. Neurosci. Res. 79:287.
  2. Ma, L. et al. (1998) Oncogene 17:1321.
  3. Kimura, I. et al. (2008) J. Biol. Chem. 283:4323.
  4. Han, K.H. et al. (2012) BMC Cancer 12:274.
  5. Kimura, I. et al. (2006) J. Neurosci. Res. 83:1415.
  6. Kimura, I. et al. (2009) Biochem. Biophys. Res. Commun. 381:75.
  7. Neubauer, H. et al. (2006) Electrophoresis 27:1840.
Entrez Gene IDs
29937 (Human); 66208 (Mouse); 289380 (Rat)
Alternate Names
Cell immortalization-related protein 2; CIR2; CIR2cell growth-inhibiting protein 47; NENF; Neudesin; neuron derived neurotrophic factor; Neuron-derived neurotrophic factor; SCIRP10; SCIRP10-related protein; Secreted protein of unknown function; Spinal cord injury related protein 10; SPUF protein; SPUF; SPUFneudesin

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