Recombinant Human Neudesin Protein, CF
Recombinant Human Neudesin Protein, CF Summary
Gly32-Phe172, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Neudesin (neuron‑derived neurotrophic secreted protein), gene name NENF (neudesin neurotrophic factor), also called CIR2 (cell immortalization‑related 2) or GIG47, is a secreted, 20‑21 kDa member of the MAPR (membrane‑associated progesterone receptor) subfamily of the cytochrome b5 family of molecules (1, 2). Human Neudesin is synthesized as a 172 amino acid (aa) precursor that contains a 31 aa signal sequence with a 141 aa mature region that possesses a cytochrome b5‑like heme‑binding domain over aa 44‑129, and a lysine acetylation site at aa 136 (1‑4). The attachment of hemin to its heme‑binding domain is necessary for its neurotrophic activity, and the binding of heme accounts for 5‑6 kDa of its circulating molecular weight (3). Mature human Neudesin shares 97%, 94% and 96% aa identity with mouse, rat, and bovine Neudesin, respectively. Neudesin appears to selectively promote cell survival or proliferation and inhibit differentiation in multiple settings. It is expressed by neuronal progenitors and neurons in the central nervous system, and by preadipocytes in white adipose tissue (5, 6). It promotes neuronal differentiation with limited proliferation and serves as a neuron survival factor, but inhibits both astrocyte and adipocyte differentiation (1, 5, 6). Neudesin over‑expression is found in tumors in the human breast, cervix, colon, lung and skin, and in human tumor cell lines that include some lymphomas and leukemias; transfection studies indicate that it may act as an oncogene (4, 7).
- Kimura, I. et al. (2005) J. Neurosci. Res. 79:287.
- Ma, L. et al. (1998) Oncogene 17:1321.
- Kimura, I. et al. (2008) J. Biol. Chem. 283:4323.
- Han, K.H. et al. (2012) BMC Cancer 12:274.
- Kimura, I. et al. (2006) J. Neurosci. Res. 83:1415.
- Kimura, I. et al. (2009) Biochem. Biophys. Res. Commun. 381:75.
- Neubauer, H. et al. (2006) Electrophoresis 27:1840.
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