Recombinant Human Neurocan Protein, CF Summary
Glu23-Cys1321, with a C-terminal 6-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Neurocan, also known as CSPG3 and 1D1, is a secreted chondroitin sulfate proteoglycan that is primarily expressed in the central nervous system (1). Human Neurocan contains one Ig-like V-type domain, two Link domains, two EGF-like domains, one C-type lectin-like domain, and one Sushi domain (2). It is an approximately 300 kDa molecule of which 66 kDa is chondroitin sulfate and 60 kDa is N‑ and O‑linked glycosylation (3). Mature human Neurocan shares 66% aa sequence identity with mature mouse and rat Neurocan. Human Neurocan is predicted to be cleaved following Met635, resulting in N-terminal (Neurocan-130) and
C-terminal (Neurocan-C) fragments whose core glycoproteins are 130 kDa and 150 kDa, respectively (4, 5). The full length molecule is expressed in the developing and juvenile brain, while its cleavage products are found throughout adulthood (3, 4). Neurocan and Neurocan-C are produced by astrocytes and accumulate in the matrix surrounding axonal bundles and neuronal cell bodies; Neurocan-130 is found mainly in the glial cell cytoplasm (6 - 8). Following brain injury, reactive astrocytes deposit increased amounts of Neurocan in the glial scar where it impedes axonal regeneration (6, 9). Neurocan inhibits neuronal adhesion and neurite outgrowth through interactions with a variety of matrix and transmembrane molecules including NCAM-L1, NCAM-1, Syndecan-3, Glypican-1, Tenascin, Contactin-2/TAG1, and HAPLN1 (1, 6, 10 - 15).
- Rauch, U. et al. (2001) Cell. Mol. Life Sci. 58:1842.
- Prange, C.K. et al. (1998) Gene 221:199.
- Rauch, U. et al. (1991) J. Biol. Chem. 266:14785.
- Matsui, F. et al. (1994) Neurochem. Int. 25:425.
- Rauch, U. et al. (1992) J. Biol. Chem. 267:19536.
- Asher, R.A. et al. (2000) J. Neurosci. 20:2427.
- Matsui, F. et al. (1998) Brain Res. 790:45.
- Abaskharoun, M. et al. (2010) Brain Res. 1327:6.
- Shen, L.H. et al. (2008) Glia 56:1747.
- Friedlander, D.R. et al. (1994) J. Cell Biol. 125:669.
- Sango, K. et al. (2003) Exp. Neurol. 182:1.
- Akita, K. et al. (2004) Biochem. J. 383:129.
- Grumet, M. et al. (1994) J. Biol. Chem. 269:12142.
- Milev, P. et al. (1996) J. Biol. Chem. 271:15716.
- Rauch, U. et al. (2004) Matrix Biol. 22:629.
Citations for Recombinant Human Neurocan Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Discovery of deoxyceramide analogs as highly selective ACER3 inhibitors in live cells
Authors: N Bielsa, M Casasamper, M Aseeri, J Casas, A Delgado, JL Abad, G Fabriàs
European Journal of Medicinal Chemistry, 2021;216(0):113296.
Sample Types: Cell Lysates
Perineuronal Net Protein Neurocan Inhibits NCAM/EphA3 Repellent Signaling in GABAergic Interneurons
Authors: CS Sullivan, I Gotthard, EV Wyatt, S Bongu, V Mohan, RJ Weinberg, PF Maness
Sci Rep, 2018;8(1):6143.
Sample Types: Whole Cells
Neurocan, an extracellular chondroitin sulfate proteoglycan, stimulates neuroblastoma cells to promote malignant phenotypes
Authors: Z Su, S Kishida, S Tsubota, K Sakamoto, D Cao, S Kiyonari, M Ohira, T Kamijo, A Narita, Y Xu, Y Takahashi, K Kadomatsu
Sample Types: Whole Cells
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