Recombinant Human Neuroligin 3/NLGN3 Protein, CF

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Recombinant Human Neuroligin 3/NLGN3 Protein, CF Bioactivity
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Product Details
Citations (1)
Supplemental Products

Recombinant Human Neuroligin 3/NLGN3 Protein, CF Summary

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human Neuroligin 3/NLGN3 is immobilized at 2 μg/mL, Biotinylated Recombinant Human Neurexin 1 beta Fc Chimera binds with a typical ED50 = 150-900 ng/mL.
Mouse myeloma cell line, NS0-derived human Neuroligin 3/NLGN3 protein
Ala39-Ser709 (Ser92Tyr), with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
76 kDa
87-101 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Bioactivity View Larger

When Recombinant Human Neuroligin 3/NLGN3 (Catalog # 9069-NL) is immobilized at 2 µg/mL, Biotinylated Recombinant Human Neurexin 1 beta Fc Chimera binds with a typical ED50 of 150-900 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Neuroligin 3/NLGN3

Neuroligin 3 (NLGN3) is one of several type I transmembrane Neuroligin proteins that play an important role in synaptic development (1, 2). Mature human Neuroligin 3 is an approximately 120 kDa molecule that consists of a 672 amino acid (aa) extracellular domain (ECD) with a catalytically inactive cholinesterase-like domain, a 21 aa transmembrane segment, and a 118 aa cytoplasmic tail (3). Alternative splicing generates additional isoforms with 20 aa or 40 aa deletions in the ECD (3-5). Within the ECD, human Neuroligin 3 shares 99% aa sequence identity with comparable regions of mouse and rat Neuroligin 3. It is expressed during development on astrocytes and Schwann cells (6) as well as on neurons where it localizes to synaptic membranes and post-synaptic densities of both glutamatergic and GABAergic synapses (7). It associates in cis with Neuroligin 1 and Neuroligin 2 and trans-synaptically with Neurexin-1 beta, -2 beta, and -3 beta that lack the splice site 4 insertion (3, 7). A soluble form of the Neuroligin 3 ECD can be released by neurons and promotes glioma cell proliferation (8). Mutations in Neuroligin 3 enhance neuronal dendritic branching, alter both excitatory and inhibitory synaptic activity (9-12), and are associated with the development of autism spectrum disorder (13, 14).

  1. Bemben, M.A. et al. (2015) Trends Neurosci. 38:496.
  2. Mackowiak, M. et al. (2014) Pharmacol. Rep. 66:830.
  3. Ichtchenko, K. et al. (1996) J. Biol. Chem. 271:2676.
  4. Philibert, R.A. et al. (2000) Gene 246:303.
  5. Talebizadeh, Z. et al. (2006) J. Med. Genet. 43:e21.
  6. Gilbert, M. et al. (2001) Glia 34:151.
  7. Budreck, E.C. and P. Scheiffele (2007) Eur. J. Neurosci. 26:1738.
  8. Venkatesh, H.S. et al. (2015) Cell 161:803.
  9. Etherton, M. et al. (2011) Proc. Natl. Acad. Sci. USA 108:13764.
  10. Foldy, C. et al. (2013) Neuron 78:498.
  11. Tabuchi, K. et al. (2007) Science 318:71.
  12. Gutierrez, R.C. et al. (2009) Neuroscience 162:208.
  13. Jamain, S. et al. (2003) Nat. Genet. 34:27.
  14. Rothwell, P.E. et al. (2014) Cell 158:198.
Entrez Gene IDs
54413 (Human)
Alternate Names
ASPGX1; AUTSX1; Gliotactin homolog; HNL3; KIAA1480HNL3; Neuroligin 3; neuroligin-3; NL3; NLGN3

Citation for Recombinant Human Neuroligin 3/NLGN3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Interaction of A? Oligomers with Neurexin 2? and Neuroligin 1 Mediates Synapse Damage and Memory Loss in Mice
    Authors: J Brito-More, MV Lourenco, MM Oliveira, JF Vital, FC Ribeiro, JH Ledo, LP Diniz, MH Magdesian, HM Melo, F Barros-Ara, JM de Souza, SV Alves-Leon, FC Gomes, JR Clarke, CP Figueiredo, FG De Felice, ST Ferreira
    J. Biol. Chem, 2017;0(0):.
    Species: Mouse
    Sample Types: Protein
    Applications: Bioassay


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