Recombinant Human Neuroligin 3/NLGN3 Protein, CF

Catalog # Availability Size / Price Qty
9069-NL-050
Recombinant Human Neuroligin 3/NLGN3 Protein, CF Bioactivity
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Recombinant Human Neuroligin 3/NLGN3 Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Neuroligin 3/NLGN3 is immobilized at 2 μg/mL, Biotinylated Recombinant Human Neurexin 1 beta Fc Chimera binds with a typical ED50 = 150-900 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human Neuroligin 3/NLGN3 protein
Ala39-Ser709 (Ser92Tyr), with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ala39
Predicted Molecular Mass
76 kDa
SDS-PAGE
87-101 kDa, reducing conditions

Product Datasheets

9069-NL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human Neuroligin 3/NLGN3 Protein, CF Bioactivity View Larger

When Recombinant Human Neuroligin 3/NLGN3 (Catalog # 9069-NL) is immobilized at 2 µg/mL, Biotinylated Recombinant Human Neurexin 1 beta Fc Chimera binds with a typical ED50 of 150-900 ng/mL.

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Background: Neuroligin 3/NLGN3

Neuroligin 3 (NLGN3) is one of several type I transmembrane Neuroligin proteins that play an important role in synaptic development (1, 2). Mature human Neuroligin 3 is an approximately 120 kDa molecule that consists of a 672 amino acid (aa) extracellular domain (ECD) with a catalytically inactive cholinesterase-like domain, a 21 aa transmembrane segment, and a 118 aa cytoplasmic tail (3). Alternative splicing generates additional isoforms with 20 aa or 40 aa deletions in the ECD (3-5). Within the ECD, human Neuroligin 3 shares 99% aa sequence identity with comparable regions of mouse and rat Neuroligin 3. It is expressed during development on astrocytes and Schwann cells (6) as well as on neurons where it localizes to synaptic membranes and post-synaptic densities of both glutamatergic and GABAergic synapses (7). It associates in cis with Neuroligin 1 and Neuroligin 2 and trans-synaptically with Neurexin-1 beta, -2 beta, and -3 beta that lack the splice site 4 insertion (3, 7). A soluble form of the Neuroligin 3 ECD can be released by neurons and promotes glioma cell proliferation (8). Mutations in Neuroligin 3 enhance neuronal dendritic branching, alter both excitatory and inhibitory synaptic activity (9-12), and are associated with the development of autism spectrum disorder (13, 14).

References
  1. Bemben, M.A. et al. (2015) Trends Neurosci. 38:496.
  2. Mackowiak, M. et al. (2014) Pharmacol. Rep. 66:830.
  3. Ichtchenko, K. et al. (1996) J. Biol. Chem. 271:2676.
  4. Philibert, R.A. et al. (2000) Gene 246:303.
  5. Talebizadeh, Z. et al. (2006) J. Med. Genet. 43:e21.
  6. Gilbert, M. et al. (2001) Glia 34:151.
  7. Budreck, E.C. and P. Scheiffele (2007) Eur. J. Neurosci. 26:1738.
  8. Venkatesh, H.S. et al. (2015) Cell 161:803.
  9. Etherton, M. et al. (2011) Proc. Natl. Acad. Sci. USA 108:13764.
  10. Foldy, C. et al. (2013) Neuron 78:498.
  11. Tabuchi, K. et al. (2007) Science 318:71.
  12. Gutierrez, R.C. et al. (2009) Neuroscience 162:208.
  13. Jamain, S. et al. (2003) Nat. Genet. 34:27.
  14. Rothwell, P.E. et al. (2014) Cell 158:198.
Entrez Gene IDs
54413 (Human)
Alternate Names
ASPGX1; AUTSX1; Gliotactin homolog; HNL3; KIAA1480HNL3; Neuroligin 3; neuroligin-3; NL3; NLGN3

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