Recombinant Human Neuroligin 3/NLGN3 Protein, CF

(1 citations)   
  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human Neuroligin 3/NLGN3 is immobilized at 2 μg/mL, Biotinylated Recombinant Human Neurexin 1 beta Fc Chimera binds with a typical ED50 = 150-900 ng/mL.
  • Source
    Mouse myeloma cell line, NS0-derived Ala39-Ser709 (Ser92Tyr), with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala39
  • Predicted Molecular Mass
    76 kDa
  • SDS-PAGE
    87-101 kDa, reducing conditions
9069-NL
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Recombinant Human Neuroligin 3/NLGN3 (Catalog # 9069-NL) is immobilized at 2 µg/mL, Biotinylated Recombinant Human Neurexin 1 beta Fc Chimera binds with a typical ED50 of 150-900 ng/mL.
Background: Neuroligin 3/NLGN3
Neuroligin 3 (NLGN3) is one of several type I transmembrane Neuroligin proteins that play an important role in synaptic development (1, 2). Mature human Neuroligin 3 is an approximately 120 kDa molecule that consists of a 672 amino acid (aa) extracellular domain (ECD) with a catalytically inactive cholinesterase-like domain, a 21 aa transmembrane segment, and a 118 aa cytoplasmic tail (3). Alternative splicing generates additional isoforms with 20 aa or 40 aa deletions in the ECD (3-5). Within the ECD, human Neuroligin 3 shares 99% aa sequence identity with comparable regions of mouse and rat Neuroligin 3. It is expressed during development on astrocytes and Schwann cells (6) as well as on neurons where it localizes to synaptic membranes and post-synaptic densities of both glutamatergic and GABAergic synapses (7). It associates in cis with Neuroligin 1 and Neuroligin 2 and trans-synaptically with Neurexin-1 beta, -2 beta, and -3 beta that lack the splice site 4 insertion (3, 7). A soluble form of the Neuroligin 3 ECD can be released by neurons and promotes glioma cell proliferation (8). Mutations in Neuroligin 3 enhance neuronal dendritic branching, alter both excitatory and inhibitory synaptic activity (9-12), and are associated with the development of autism spectrum disorder (13, 14).
  • References:
    1. Bemben, M.A. et al. (2015) Trends Neurosci. 38:496.
    2. Mackowiak, M. et al. (2014) Pharmacol. Rep. 66:830.
    3. Ichtchenko, K. et al. (1996) J. Biol. Chem. 271:2676.
    4. Philibert, R.A. et al. (2000) Gene 246:303.
    5. Talebizadeh, Z. et al. (2006) J. Med. Genet. 43:e21.
    6. Gilbert, M. et al. (2001) Glia 34:151.
    7. Budreck, E.C. and P. Scheiffele (2007) Eur. J. Neurosci. 26:1738.
    8. Venkatesh, H.S. et al. (2015) Cell 161:803.
    9. Etherton, M. et al. (2011) Proc. Natl. Acad. Sci. USA 108:13764.
    10. Foldy, C. et al. (2013) Neuron 78:498.
    11. Tabuchi, K. et al. (2007) Science 318:71.
    12. Gutierrez, R.C. et al. (2009) Neuroscience 162:208.
    13. Jamain, S. et al. (2003) Nat. Genet. 34:27.
    14. Rothwell, P.E. et al. (2014) Cell 158:198.
  • Entrez Gene IDs:
    54413 (Human)
  • Alternate Names:
    ASPGX1; AUTSX1; Gliotactin homolog; HNL3; KIAA1480HNL3; Neuroligin 3; neuroligin-3; NL3; NLGN3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Interaction of A? Oligomers with Neurexin 2? and Neuroligin 1 Mediates Synapse Damage and Memory Loss in Mice
    Authors: J Brito-More, MV Lourenco, MM Oliveira, JF Vital, FC Ribeiro, JH Ledo, LP Diniz, MH Magdesian, HM Melo, F Barros-Ara, JM de Souza, SV Alves-Leon, FC Gomes, JR Clarke, CP Figueiredo, FG De Felice, ST Ferreira
    J. Biol. Chem, 2017;0(0):.
    Species: Mouse
    Sample Type: Protein
    Application: Bioassay
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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