Recombinant Human Nogo-A (aa 566-748) Fc Chimera Protein, CF

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3515-NG-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human Nogo-A (aa 566-748) Fc Chimera Protein, CF Summary

Product Specifications

Purity
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 200 ng on a nitrocellulose-coated microplate.
Source
Chinese Hamster Ovary cell line, CHO-derived human Nogo-A protein
Human Nogo-A
(Val566 - Phe748) &
(Thr569 - Phe748)
Accession # Q9NQC3
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Val566 & Thr569
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
46 kDa (monomer)
SDS-PAGE
70-90 kDa, reducing conditions

Product Datasheets

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3515-NG

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3515-NG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Nogo-A

Human Nogo-A (also reticulon-4) is a member of the reticulon family of transmembrane proteins. This family is characterized by the presence of a nonsignal sequence-containing N-terminus, a topologically conserved 200 amino acid (aa) C-terminus that contains two transmembrane domains with an ER-retention motif, and a punctate intracellular distribution within the ER that is reminescent of a reticulum (1 - 4). In human, Nogo exists in five isoforms (5 - 7). The full length human form (Nogo-A) is 1192 aa in length and contains a 1018 aa N-terminus, a 21 aa transmembrane segment, a 94 aa connecting “loop”, a second 21 aa transmembrane segment, and a 38 aa C-terminus. Three areas are of particular interest. One is a stretch of 66 aa within the 94 aa connecting loop. This segment is reported to bind to the GPI-linked Nogo receptor/p75 complex on axons and induce growth cone collapse (8 - 10). Two other areas in the N-terminus have also been discovered to have bioactivity (8, 11, 12). Based on rat, aa 57 - 184 in human (aa 59 - 172 in rat) should block fibroblast spreading, while aa 566 - 748 in human (aa 544 - 725 in rat) block neurite outgrowth and block fibroblast spreading (8, 12, 13). The exact topology of Nogo-A is unclear. The N- and C-termini may be extracellular with the “loop” region intracellular, or the situation could be reversed (13 - 15). Alternatively, the loop region and N-terminus may be on the same side of the membrane (3, 8). The four additional isoforms are shorter than Nogo-A (199 aa [Nogo-C], 373 aa [Nogo-B], 392 aa and 986 aa, respectively) (7). Although highly divergent, all contain the same C-terminal stretch, aa 1005 - 1192. Both Nogo-B and C are reported to complex with Nogo-A (16). Notably, Nogo-A is expressed in neurons, endothelial cells. oligodendrocytes, fibroblasts and myoblasts (12, 16 - 18). Human Nogo-A is 78% aa identical to mouse and rat Nogo-A overall, with 98% aa identity in the loop region and approximately 80% aa identity in the aa 566 - 748 segment.

References
  1. Oertle, T. et al. (2003) FASEB J. 17:1238.
  2. GrandPre, T. et al. (2000) Nature 403:439.
  3. Yan, R. et al. (2006) Cell. Mol. Life Sci. 63:877.
  4. Teng, F.Y.H. et al. (2004) J. Neurochem. 89:801.
  5. Chen, M.S. et al. (2000) Nature 403:434.
  6. Morris, N.J. et al. (1999) Biochim. Biophys. Acta 1450:68.
  7. GenBank Accession # Q9NQC3.
  8. Oertle, T. et al. (2003) J. Neurosci. 23:5393.
  9. Fournier, A.E. et al. (2001) Nature 409:341.
  10. Wang, K.C. et al. (2002) Nature 420:74.
  11. Prinjha, R. et al. (2000) Nature 403:384.
  12. Dodd, D.A. et al. (2005) J. Biol. Chem. 280:12494.
  13. Li, M. et al. (2004) Eur. J. Biochem. 271:3512.
  14. Huber, A.B. and M.E. Schwab (2000) Biol. Chem. 381:407.
  15. Ng, C.E.L. and B.L. Tang (2002) J. Neurosci. Res. 67:559.
  16. Dodd, D.A. et al. (2005) J. Biol. Chem. 280:12494.
  17. Wang, X. et al. (2002) J. Neurosci. 22:5505.
  18. Acevedo, L. et al. (2004) Nat. Med. 10:382.
Long Name
Reticulon 4A
Entrez Gene IDs
57142 (Human); 68585 (Mouse); 83765 (Rat)
Alternate Names
ASY;Nbla00271;Nbla10545;NI220/250;Nogo;NSP;NSP-CL;r;Reticulon-4;Rtn4;RTN4;RTN4-A;RTN4-B1;RTN4-B2;RTN4-C;RTN-X; NI220; NogoA; Nogo-A; RTN4; RTN4A

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Recombinant Human Nogo-A (aa 566-748) Fc Chimera Protein, CF
By Anonymous on 08/18/2021
Application: Binding assay/Protein-protein interaction