Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain, CF

(30 citations)   
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.06-0.3 ng/mL.
  • Source
    E. coli-derived Thr176-Lys246
  • Accession #
  • N-terminal Sequence
    Analysis
    Thr176
  • Predicted Molecular Mass
    8 kDa
  • SDS-PAGE
    8 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
396-HB/CF
 
396-HB
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain (Catalog # 396-HB/CF) stimulates cell proliferation of the MCF-7 human breast cancer cell line. The ED50 for this effect is 0.06-0.3 ng/mL.
1 μg/lane of Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 8 kDa.
Background: Neuregulin-1 beta 1/NRG1 beta 1

The neuregulin family of structurally related glycoproteins comprises products from four distinct but related genes, Nrg-1, Nrg-2, Nrg-3, and Nrg-4. Through alternative splicing or the use of alternative promoters, Nrg-1 has been shown to encode more than 14 soluble or transmembrane proteins. The extracellular domain of the transmembrane NRG1 isoforms can be proteolytically cleaved to release soluble growth factors. All NRG1 isoforms contain an EGF-like domain ( alpha - or beta -splice variant that differ in their C-terminal region) that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. The ErbB3 or ErbB4 subsequently recruits and heterodimerizes with ErbB2, resulting in tyrosine phosphorylation and NRG1 signaling. NRG1 isoforms can be classified into three major subtypes. Type I (Neu Differentiation Factor, NDF; Heregulin, HRG; Acetylcholine Receptor Inducing Activity, ARIA) and type II (Glial Growth Factor, GGF) NRG1s have an immunoglobulin (Ig)-like domain N-terminal to the EGF-like domain. Type I NRG1s differ from type II NRG1s by having a glycosylation-rich domain between the Ig-like and the EGF-like domains. Type III NRG1s (Sensory and Motor neuron-Derived Factor) lacks the Ig-like domain but has a cysteine rich domain (CRD) instead. NRG1 isoforms show distinct spatial and temporal expression patterns. These proteins play important roles during development of both the nervous system and the heart. They have been shown to regulate the selective expression of neurotransmitter receptors in neurons and at the neuromuscular junction, and promote the differentiation and development of Schwann cells from neural crest stem cells. NRG1s have also been shown to be involved in the establishment of the oligodendroglial lineage.

  • References:
    1. Buonanno, A., and Fischbach, G.D. (2001) Curr. Opin. Neurobiol. 11:287.
    2. Adlkofer, K. and Lai, C. (2000) Glia 29:104.
    3. Garratt, A.N. et al. (2000) BioEssays 22:987.
  • Entrez Gene IDs:
    3084 (Human); 211323 (Mouse); 112400 (Rat)
  • Alternate Names:
    Neuregulin1 beta 1; Neuregulin-1 beta 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Remyelination improvement after neurotrophic factors secreting cells transplantation in rat spinal cord injury
    Authors: S Razavi, N Ghasemi, M Mardani, H Salehi
    Iran J Basic Med Sci, 2017;20(4):392-398.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Developmental pruning of excitatory synaptic inputs to parvalbumin interneurons in monkey prefrontal cortex
    Authors: DW Chung, ZP Wills, KN Fish, DA Lewis
    Proc. Natl. Acad. Sci. U.S.A, 2017;114(4):E629-E637.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. A xenogeneic-free system generating functional human gut organoids from pluripotent stem cells
    Authors: H Uchida, M Machida, T Miura, T Kawasaki, T Okazaki, K Sasaki, S Sakamoto, N Ohuchi, M Kasahara, A Umezawa, H Akutsu
    JCI Insight, 2017;2(1):e86492.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Heregulin-1� and HER3 in hepatocellular carcinoma: status and regulation by insulin
    J Exp Clin Cancer Res, 2016;35(1):126.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. HRG/HER2/HER3 signaling promotes AhR-mediated Memo-1 expression and migration in colorectal cancer
    Oncogene, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Myelinating glia differentiation is regulated by extracellular matrix elasticity
    Sci Rep, 2016;6(0):33751.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  7. Regulation of inflammatory responses by neuregulin-1 in brain ischemia and microglial cells in vitro involves the NF-kappa B pathway
    J Neuroinflammation, 2016;13(1):237.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  8. Activation of endothelial ?-catenin signaling induces heart failure
    Sci Rep, 2016;6(0):25009.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  9. Zeb2 recruits HDAC-NuRD to inhibit Notch and controls Schwann cell differentiation and remyelination
    Authors: Lai Man Natalie Wu
    Nat Neurosci, 2016;0(0):.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  10. The reemergence of long-term potentiation in aged Alzheimer's disease mouse model
    Sci Rep, 2016;6(0):29152.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  11. Label-free imaging of Schwann cell myelination by third harmonic generation microscopy.
    Authors: Lim H, Sharoukhov D, Kassim I, Zhang Y, Salzer J, Melendez-Vasquez C
    Proc Natl Acad Sci U S A, 2015;111(0):18025.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  12. Neuregulin-1 inhibits neuroinflammatory responses in a rat model of organophosphate-nerve agent-induced delayed neuronal injury.
    Authors: Li Y, Lein P, Ford G, Liu C, Stovall K, White T, Bruun D, Tewolde T, Gates A, Distel T, Surles-Zeigler M, Ford B
    J Neuroinflammation, 2015;12(1):64.
    Species: Rat
    Sample Type: In Vivo
  13. Basal cell signaling by p63 controls luminal progenitor function and lactation via NRG1.
    Authors: Forster N, Saladi S, van Bragt M, Sfondouris M, Jones F, Li Z, Ellisen L
    Dev Cell, 2014;28(2):147-60.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  14. Peripheral nervous system plasmalogens regulate Schwann cell differentiation and myelination.
    Authors: Da Silva T, Eira J, Lopes A, Malheiro A, Sousa V, Luoma A, Avila R, Wanders R, Just W, Kirschner D, Sousa M, Brites P
    J Clin Invest, 2014;124(6):2560-70.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  15. Transient overexposure of neuregulin 3 during early postnatal development impacts selective behaviors in adulthood.
    Authors: Paterson, Clare, Law, Amanda J
    PLoS ONE, 2014;9(8):e104172.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  16. The broad-spectrum metalloproteinase inhibitor BB-94 inhibits growth, HER3 and Erk activation in fulvestrant-resistant breast cancer cell lines.
    Authors: Kirkegaard T, Yde C, Kveiborg M, Lykkesfeldt A
    Int J Oncol, 2014;45(1):393-400.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. Targeting ErbB-2 nuclear localization and function inhibits breast cancer growth and overcomes trastuzumab resistance.
    Authors: Cordo Russo R, Beguelin W, Diaz Flaque M, Proietti C, Venturutti L, Galigniana N, Tkach M, Guzman P, Roa J, O'Brien N, Charreau E, Schillaci R, Elizalde P
    Oncogene, 2014;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  18. Recombinant neuregulin 1 does not activate cardiomyocyte DNA synthesis in normal or infarcted adult mice.
    Authors: Reuter S, Soonpaa M, Firulli A, Chang A, Field L
    PLoS ONE, 2014;9(12):e115871.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  19. Dual cleavage of neuregulin 1 type III by BACE1 and ADAM17 liberates its EGF-like domain and allows paracrine signaling.
    Authors: Fleck D, van Bebber F, Colombo A, Galante C, Schwenk B, Rabe L, Hampel H, Novak B, Kremmer E, Tahirovic S, Edbauer D, Lichtenthaler S, Schmid B, Willem M, Haass C
    J Neurosci, 2013;33(18):7856-69.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  20. An efficient method for dorsal root ganglia neurons purification with a one-time anti-mitotic reagent treatment.
    Authors: Liu, Rui, Lin, Gou, Xu, Hanpeng
    PLoS ONE, 2013;8(4):e60558.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  21. The MAPK pathway is a predominant regulator of HLA-A expression in esophageal and gastric cancer.
    Authors: Mimura, Kousaku, Shiraishi, Kensuke, Mueller, Anja, Izawa, Shinichi, Kua, Ley-Fang, So, Jimmy, Yong, Wei-Peng, Fujii, Hideki, Seliger, Barbara, Kiessling, Rolf, Kono, Koji
    J Immunol, 2013;191(12):6261-72.
    Species: Human
    Sample Type: Whole Cells
    Application: cell culture
  22. Cellular Effects of HER3-Specific Affibody Molecules.
    Authors: Gostring L, Malm M, Hoiden-Guthenberg I, Frejd FY, Stahl S, Lofblom J, Gedda L
    PLoS ONE, 2012;7(6):e40023.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  23. Subchronic peripheral neuregulin-1 increases ventral hippocampal neurogenesis and induces antidepressant-like effects.
    Authors: Mahar I, Tan S, Davoli MA, Dominguez-Lopez S, Qiang C, Rachalski A, Turecki G, Mechawar N
    PLoS ONE, 2011;6(10):e26610.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  24. Neuregulin-1 regulates LTP at CA1 hippocampal synapses through activation of dopamine D4 receptors.
    Authors: Kwon OB, Paredes D, Gonzalez CM, Neddens J, Hernandez L, Vullhorst D, Buonanno A
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(40):15587-92.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  25. Human epidermal growth factor receptor (HER-1:HER-3) Fc-mediated heterodimer has broad antiproliferative activity in vitro and in human tumor xenografts.
    Authors: Sarup J, Jin P, Turin L, Bai X, Beryt M, Brdlik C, Higaki JN, Jorgensen B, Lau FW, Lindley P, Liu J, Ni I, Rozzelle J, Kumari R, Watson SA, Zhang J, Shepard HM
    Mol. Cancer Ther., 2008;7(10):3223-36.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  26. Identification of neuregulin as a factor required for formation of aligned spermatogonia.
    Authors: Hamra FK, Chapman KM, Nguyen D, Garbers DL
    J. Biol. Chem., 2007;282(1):721-30.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  27. Central administration of transforming growth factor-alpha and neuregulin-1 suppress active behaviors and cause weight loss in hamsters.
    Authors: Snodgrass-Belt P, Gilbert JL, Davis FC
    Brain Res., 2005;1038(2):171-82.
    Species: Hamster
    Sample Type: In Vivo
    Application: In Vivo
  28. Prolactin and heregulin override DNA damage-induced growth arrest and promote phosphatidylinositol-3 kinase-dependent proliferation in breast cancer cells.
    Authors: Chakravarti P, Henry MK, Quelle FW
    Int. J. Oncol., 2005;26(2):509-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  29. Neuregulin-1 is neuroprotective and attenuates inflammatory responses induced by ischemic stroke.
    Authors: Xu Z, Jiang J, Ford G, Ford BD
    Biochem. Biophys. Res. Commun., 2004;322(2):440-6.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  30. Heregulin ameliorates the dystrophic phenotype in mdx mice.
    Authors: Krag TO, Bogdanovich S, Jensen CJ, Fischer MD, Hansen-Schwartz J, Javazon EH, Flake AW, Edvinsson L, Khurana TS
    Proc. Natl. Acad. Sci. U.S.A., 2004;101(38):13856-60.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
Expand to show all 30 Citations

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