Recombinant Human OCIL/CLEC2d Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10506-OC-050
Recombinant Human OCIL/CLEC2d Fc Chimera Protein Binding Activity.
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Recombinant Human OCIL/CLEC2d Fc Chimera Protein, CF Summary

Product Specifications

Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD161 Fc Chimera Protein (Catalog # 7448-CD) is immobilized at 10 μg/mL (100 μL/well), the concentration of Recombinant Human OCIL/CLEC2d Fc Chimera that produces 50% of the optimal binding response is approximately 1.5-9 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human OCIL/CLEC2d protein
MDHuman IgG1
(Pro100-Lys330)
IEGRHuman OCIL/CLEC2d
(Ala58-Val191)
Accession # Q9UHP7.1
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis

Met

Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
42 kDa
SDS-PAGE
50-60 kDa, under reducing conditions

Product Datasheets

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10506-OC

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10506-OC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When Recombinant Human CD161 Fc Chimera Protein (7448-CD) is immobilized at 10 μg/mL (100 μL/well), the concentration of Recombinant Human OCIL/CLEC2d Fc Chimera (Catalog # 10506-OC) that produces 50% of the optimal binding response is approximately 1.5-9 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: OCIL/CLEC2d

C-type lectin domain family 2 member D (CLEC2D), also known as lectin-like NK cell receptor,as lectin-like transcript 1 (LLT-1) and osteoclast inhibitory lectin (OCIL), is a member of the C-type lectin like (CTL) superfamily of natural killer cell receptors (1, 2). Human OCIL/CLEC2D is highly glycosylated and consists of a cytoplasmic domain, a type II transmembrane domain, a stalk region and a C-type lectin-like (CTL) in the extracellular domain (ECD). Within the ECD, mature human OCIL/CLEC2D shares 45% and 43% amino acid sequence identity with mouse and rat OCIL/CLEC2D, respectively. At least six different isoforms of OCIL/CLEC2D, resulting from alternatively spicing, have been reported (1, 2). OCIL/ CLEC2D plays a role in immunotherapy, inflammation and tissue injury and is expressed mainly on activated lymphocytes (NK cells, T cells, B cells) and antigen presenting cells (1-6).OCIL/CLEC2D forms a disulfide linked homodimer that acts as the only ligand for NKRP1A (CD161) and inhibits NK cell-mediated cytotoxicity and IFN-gamma secretion (1,6,7). OCIL/CLEC2D preferentially binds high molecular weight sulfated glycosaminoglycans and blocks osteoclast differentiation (1, 4).

References
  1. Marrufo, A. et al. (2018) Am. J. Cancer Res. 8:1050.
  2. Germain, C. et al. (2010) J. Biol. Chem. 285:36207.
  3. Mathew, S. et al. (2016) Oncotarget 7:68650.
  4. Lai, J. et al. (2020) Immunity 52:123.
  5. Varaden, D. et al. (2019) Eur. J. Obstet. Gynecol. Reprod. Biol. X. 3:100039.
  6. Sun, Y. et al. (2019) J. Cancer Metastasis Treat. 5:80.
  7. Skalova, T. et al. (2015) Acta. Crystallogr. D. Biol. Crystallogr. 71:578.
Long Name
Osteoclast Inhibitory Lectin
Entrez Gene IDs
29121 (Human); 93694 (Mouse)
Alternate Names
CLAXLLT-1; CLEC2D; C-type lectin domain family 2 member D; C-type lectin domain family 2, member D; C-type lectin related f; Lectin-like NK cell receptor; Lectin-like transcript 1; LLT1; LLT1Osteoclast inhibitory lectin; OCIL; OCILmember D

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