Recombinant Human OSCAR Fc Chimera Protein, CF

R&D Systems | Catalog # 9955-OS

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human OSCAR Fc Chimera Protein (9955-OS)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

NP_573399

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all OSCAR Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human OSCAR protein
Human OSCAR
(Asp19-Asn229)
Accession # NP_573399		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp19

Predicted Molecular Mass

50 kDa

SDS-PAGE

61-70 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Bovine Collagen I is coated at 10 µg/mL, Recombinant Human OSCAR Fc Chimera binds with an ED50 of 1-8 ng/mL.

Scientific Data Images for Recombinant Human OSCAR Fc Chimera Protein, CF

Recombinant Human OSCAR Fc Chimera Protein Binding Activity

Recombinant Human OSCAR Fc Chimera Protein Binding Activity

When Bovine Collagen I is coated at 10 µg/mL, Recombinant Human OSCAR Fc Chimera binds with an ED50 of 1-8 ng/mL.
Recombinant Human OSCAR Fc Chimera Protein SDS-PAGE

Recombinant Human OSCAR Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human OSCAR was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 61-70 kDa and 120-140 kDa, respectively.

Formulation, Preparation, and Storage

9955-OS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

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Background: OSCAR

OSCAR (Osteoclast-associated immunoglobulin-like receptor), also known as PIgR-3 (polymeric immunoglobulin-like receptor), is a type I transmembrane protein of the leukocyte receptor complex (LRC) family (1). The mature human OSCAR includes an extracellular domain (ECD) with two Ig domains homology to PIR family proteins, a transmembrane region, and a cytoplasmic domain. The ECD of human OSCAR shares 77% and 72% amino acid sequence identity with the ECD of mouse and rat OSCAR, respectively. OSCAR is specifically expressed by pre-osteoclasts and signals via FcR gamma (2). OSCAR co-stimulates one of the major FcR gamma -associated pathways required for osteoclastogenesis in vivo (2). Collagen type I and collagen type II serve as ligands for OSCAR (3). OSCAR binds to specific motifs within fibrillary collagens in the extracellular matrix (ECM) (2). OSCAR promoted osteoclastogenesis in vivo, and its binding to the collagen motif led to signaling that increased numbers of osteoclasts in culture (2). OSCAR contains two immunoglobulin-like domains, D1 and D2. Direct binding assays on OSCAR molecules showed that D2, but not D1, is critical for collagen binding (4). OSCAR may contribute to the pathogenesis and severity of a number of diseases including osteoporosis, atherosclerosis, chronic obstructive pulmonary disease and rheumatoid arthritis (4).

References

  1. Kim N. el al. (2002) J. Exp. Med. 195:201.
  2. Barrow, A.D. et al. (2011) J. Clin. Invest. 121:3505.
  3. Shultz, H.S. et al. (2016) Eur. J. Immunol. 46:952.
  4. Zhou, L. et al. (2016) Blood 127:529.

Long Name

Osteoclast-associated Receptor

Alternate Names

hOSCAR, MGC33613, osteoclast associated receptor OSCAR-S1, osteoclast associated receptor OSCAR-S2, osteoclast associated, immunoglobulin-like receptor, osteoclast-associated immunoglobulin-like receptor, Osteoclast-associated receptor, PIGR3, PIgR-3, Poly-Ig receptor 3, Polymeric immunoglobulin receptor 3

Entrez Gene IDs

126014 (Human); 232790 (Mouse)

Gene Symbol

OSCAR

UniProt

NP_573399

Additional OSCAR Products

Product Documents for Recombinant Human OSCAR Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human OSCAR Fc Chimera Protein, CF

For research use only

Related Research Areas

Osteoblast and Osteoclast Markers

Citations for Recombinant Human OSCAR Fc Chimera Protein, CF

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