Recombinant Human P4HB Protein, CF

Catalog # Availability Size / Price Qty
4236-DI-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human P4HB Protein, CF Summary

Product Specifications

Purity
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to promote aggregation of insulin in the presence of DTT. The specific activity is >7.5 A650/cm/min/mg, as measured under the described conditions.
Source
Chinese Hamster Ovary cell line, CHO-derived human Protein Disulfide Isomerase/P4HB protein
Asp18-Lys505, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Asp18
Predicted Molecular Mass
56 kDa
SDS-PAGE
60 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4236-DI

Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Protein Disulfide Isomerase/P4HB

Protein Disulfide Isomerase, also known as prolyl 4‑hydroxylase subunit beta (P4HB), protocollagen hydroxylase, cellular thyroid hormone binding protein p55 and glutathione-insulin transhydrogenase (1-3) is an abundant multifunctional enzyme that belongs to the Protein Disulfide Isomerase family. It contains two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. When present as a tetramer consisting of two alpha subunits and two beta subunits, this enzyme functions as a hydroxylase catalyzing the hydroxylation of prolyl residues in preprocollagen. P4HB has various additional functions (4-7). It binds thyroid hormone. It acts as a chaperone that inhibits aggregation of misfolded proteins. It plays a role in both the influx and efflux of S-nitrosothiol-bound nitric oxide. It is also a subunit of the microsomal triglyceride transfer protein complex.

References
  1. Pihlajaniemi, T. et al. (1987) EMBO J. 6:643.
  2. Cheng, S.Y. et al. (1987) J. Biol. Chem. 262:11221.
  3. Morris, J. I. and Varandani, P. T. (1988) Biochim. Biophys. Acta 949:169.
  4. Obata, T. et al. (1988) J. Biol. Chem. 263:782.
  5. Gilbert, H. F. (1997) J. Biol. Chem. 272:29399.
  6. Sliskovic, I. et al. (2005) J. Biol. Chem. 280:8733.
  7. Wetterau, J. R. et al. (1990) J. Biol. Chem. 265:9800.
Long Name
Proline 4-Hydroxylase, Beta Polypeptide
Entrez Gene IDs
5034 (Human); 18453 (Mouse); 25506 (Rat)
Alternate Names
Cellular thyroid hormone-binding protein; collagen prolyl 4-hydroxylase beta; DSI; ERBA2L; GIT; glutathione-insulin transhydrogenase; P4HB; P4Hbeta; p55; PDI; PDIA1; PDIA1procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase-associated 1); PDIEC 5.3.4.1; PHDB; PO4DB; PO4HB; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide; PROHB; PROHBprocollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase; thyroid hormone binding protein p55); Prolyl 4-hydroxylase subunit beta; prolyl 4-hydroxylase, beta polypeptide; protein disulfide isomerase family A, member 1; protein disulfide isomerase/oxidoreductase; protein disulfide isomerase-associated 1; protein disulfide-isomerase; protocollagen hydroxylase; Thbp; thyroid hormone-binding protein p55

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