Recombinant Human PGPEP-1 Protein, CF
R&D Systems | Catalog # 6278-CY
Key Product Details
- R&D Systems E. coli-derived Recombinant Human PGPEP-1 Protein (6278-CY)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Applications
Product Specifications
Source
Met1-His209, with a C-terminal 6-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The specific activity is >9,500 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation, and Storage
6278-CY
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and DTT. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: PGPEP-1
References
- Kilbane Z. et al. (2007) Mol. Cell. Biochem. 297:189.
- Cummins P.M. and B. O'Connor (1998) Biochim. Biophys. Acta. 1429:1.
- Dando P.M. et al. (2003) Protein Express. Purif. 28:111.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional PGPEP-1 Products
Product Documents for Recombinant Human PGPEP-1 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human PGPEP-1 Protein, CF
Coomassie is a registered trademark of Imperial Chemical Industries Ltd.
For research use only
Related Research Areas
Citations for Recombinant Human PGPEP-1 Protein, CF
Powered by Bioz
Customer Reviews for Recombinant Human PGPEP-1 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human PGPEP-1 Protein, CF and earn rewards!
Have you used Recombinant Human PGPEP-1 Protein, CF?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
View specific protocols for Recombinant Human PGPEP-1 Protein, CF (6278-CY):
- Assay Buffer: 50 mM Tris, 5 mM DTT, pH 9.0
- Recombinant Human PGPEP-1 (rhPGPEP-1) (Catalog # 6278-CY)
- Substrate: L-pyroglutamic acid-AMC (Pyr-AMC) (Bachem, Catalog # I-1300), 10 mM stock in DMSO
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
1. Dilute rhPGPEP-1 to 0.1 ng/μL in Assay Buffer.
2. Dilute Substrate to 100 μM in Assay Buffer.
3. Load into a plate 50 μL of 0.1 ng/μL rhPGPEP-1, and start the reaction by adding 50 μL of 100 μM Substrate. For Substrate Blanks, load 50 μL of Assay Buffer and 50 μL of 100 μM Substrate.
4. Read plate at excitation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes.
5. Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard 7-Amino, 4-Methyl Coumarin (Sigma, Catalog # A-9891).
- rhPGPEP-1: 0.005 μg
- Substrate: 50 μM
FAQs for Recombinant Human PGPEP-1 Protein, CF
-
Q: Do you have a unit conversion for pmol/min/μg to units?
A: Conversion to International Units would require testing our enzyme directly side-by-side with a biological reference material from NIBSC. The NIBSC catalog does not currently have any reference materials for the PGPEP-1 protein, so this is not currently possible.
Additionally, the assay to measure activity of this protein involves reading fluorescence of the fluorogenic peptide substrate in kinetic mode. Since we're reading in kinetic mode, we're specifically interested in the change in fluorescence over time. That means that time is an integral part of the pmol/min/μg units.