Recombinant Human Plexin A1 His-tag Protein, CF Summary
Glu27 - Pro1244, with a C-terminal 6-His tag
Complete Your Research
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Supplied as a 0.2 μm filtered solution in PBS.|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
2 μg/lane of Recombinant Human Plexin A1 His-tag (Catalog # 10167-PA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 130-150 kDa...
Background: Plexin A1
Plexin A1, also known as Plexin 1, is a type I transmembrane protein that is a member of the Plexin family of Semaphorin signal transducers (1). In humans, the Plexin family consists of at least nine members divided into four subfamilies and Plexin signaling is involved in neuronal axon guidance, angiogenesis and immune response (2). Mature human Plexin A1 consists of an extracellular domain (ECD) with one Sema domain, a spacer, and four tandem IPT/TIG domains, a transmembrane segment, and a large cytoplasmic domain. Within the ECD, human Plexin A1 shares 95% amino acid sequence identity with mouse and rat Plexin A1. Plexin A1 binds Class 3 (secreted) Semaphorins indirectly via Neuropilin (Npn)-1 and Npn-2 and binds transmembrane Semaphorin 6D directly (3 ‑ 5). Sema3A engagement of Plexin A1 and Npn-1 guides proprioceptive and sensory neurons during development, while Sema3B engagement guides floorplate neurons (5-8). In contrast, T cell Sema6D engagement of dendritic cell Plexin A1 controls actin polymeration, which supports formation of immunological synapses and enhances the function of the dendritic cells (3, 4, 9). Complex formation with DAP12 allows Plexin A1 signaling through TREM family proteins (10, 11). However, the most striking effect of Plexin A1 deletion is on bone homeostasis, where Plexin A1-deficient mice show increased trabecular bone mass due to downregulated osteoclast differentiation (10). Plexin A1 and Sema6D are frequently expressed in malignant pleural mesothelioma, where they promote anchorage-independent growth through complexing with and activating VEGF R2 (12). Plexin A1 has also been identified as a receptor for Slits and mediate commissural growth cone collapse (13).
- Kameyama, T. et al. (1996) Biochem. Biophys. Res. Commun. 226:524.
- Kruger, R.P. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:789.
- Takamatsu, H. et al. (2010) Cell. Mol. Immunol. 7:83.
- O’Connor, B.P. and J.P.Y. Ting (2008) Immunol. Res. 41:217.
- Takahashi, T. et al. (1999) Cell 99:59.
- Yoshida, Y. et al. (2006) Neuron 52:775.
- Toyofuku, T. et al. (2005) Nat. Neurosci. 8:1712.
- Nawabi, H. et al. (2010) Genes Dev. 24:396.
- Eun, S-Y. et al. (2006) J. Immunol. 177:4271.
- Takegahara, N. et al. (2006) Nat. Cell Biol. 8:615.
- Watarai, H. et al. (2008) Proc. Natl. Acad. Sci. USA 105:2993.
- Catalano, A. et al. (2009) Cancer Res. 69:1485.
- Delloye-Bourgeois, C. et al. (2015) Nat Neurosci 18:36.
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Human Plexin A1 His-tag Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human Plexin A1 His-tag Protein, CF and earn rewards!
Have you used Recombinant Human Plexin A1 His-tag Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image