Recombinant Human PLUNC BPIFA1 His-tag Protein, CF

R&D Systems | Catalog # 11221-BF

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems HEK293-derived Recombinant Human PLUNC BPIFA1 His-tag Protein (11221-BF)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

Q9NP55.1

Applications

Bioactivity
View all PLUNC Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human PLUNC protein
Gln20-Val256, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln20; deblocked shows Phe21

Predicted Molecular Mass

25 kDa

SDS-PAGE

21-28 kDa, under reducing conditions.

Activity

Measured by its ability to bind fluorescein conjugated E. coli bio-particles. The ED50 for this effect is 0.500-5.00 μg/mL.

Scientific Data Images for Recombinant Human PLUNC BPIFA1 His-tag Protein, CF

Recombinant Human PLUNC BPIFA1 His-tag Protein Binding Activity.

Measured by its ability to bind fluorescein conjugated E. coli bio-particles. The ED50 for this effect is 0.500-5.00 μg/mL.

Recombinant Human PLUNC BPIFA1 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human PLUNC BPIFA1 His-tag Protein (Catalog # 11221-BF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 21-28 kDa.

Formulation, Preparation, and Storage

11221-BF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: PLUNC

BPIFA1 (BPI fold-containing family A member 1), also named SPLUNC1, is a member of the BPI-fold (BPIF) containing/Plunc (palate, lung, and nasal epithelium clone) superfamily of putative innate defense molecules which are predominantly expressed in regions of the oral cavity, nasopharynx, and upper respiratory tract (1, 2). BPIF proteins exist as two subgroups, BPIFA (formally SPLUNCs) and BPIFB (formally LPLUNCs) (1, 3). BPIFA proteins have structural homology to the N‑terminal domain of BPI whereas BPIFB proteins have structural homology to both domains of BPI (2). The mature human BPIFA1 shares 68% amino acid (aa) identity with mouse and rat BIPFA1. BPIF proteins appear to exhibit distinct tissue and cell specific expression patterns with various family members, being localized to several glandular structures within the upper respiratory tract, nasopharyngeal regions and oral cavity where they are secreted from these tissues and are found in high levels in saliva and nasal and respiratory lining fluids (2). BPI proteins play a role in diverse functions, including neutralization of endotoxin (LPS) in septic shock patients, inhibition of endothelial cell growth, dendritic cell maturation, and function as an anti-angiogenic, chemoattractant or opsonization agent (2). BPIFA1 and BPIFB1 expression was increased in late-stage chronic obstructive pulmonary disease (COPD) patients, and elevated levels correlate with disease severity (4). BPIFs are also upregulated in cystic fibrosis (CF) lung disease and may play a role in the pathogenesis of the disease (5).

References

  1. Bingle, C.D. and C. J. Craven (2002) Hum. Mol. Genet. 11:937.
  2. Alves, D.B. et al. (2017) Braz. Oral Res. 31:e6.
  3. Bingle, L. et al. (2012) Histochem. Cell Biol. 138:749.
  4. De Smet, E.G. et al. (2017) Int. J. Chron. Obstruct. Pulmon. Dis. 13:11.
  5. Saferali, A. et al. (2015) Am. J. Respir. Cell. Mol. Biol. 53:607.

Long Name

Palate, Lung, and Nasal Epithelium Associated

Alternate Names

BPIFA1, LUNX, SPLUNC1

Entrez Gene IDs

51297 (Human)

Gene Symbol

BPIFA1

UniProt

Q9NP55.1

Additional PLUNC Products

Product Documents for Recombinant Human PLUNC BPIFA1 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human PLUNC BPIFA1 His-tag Protein, CF

For research use only

Customer Reviews for Recombinant Human PLUNC BPIFA1 His-tag Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human PLUNC BPIFA1 His-tag Protein, CF and earn rewards!

Have you used Recombinant Human PLUNC BPIFA1 His-tag Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes