Recombinant Human Podocin/NPHS2 Protein, CF

Catalog # Availability Size / Price Qty
9287-PO-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human Podocin/NPHS2 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When recombinant human Nephrin is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human Podocin/NPHS2 binds with a typical ED50 of 0.8-4 μg/mL.
Source
E. coli-derived human Podocin/NPHS2 protein
Lys259-Leu383, with an N-terminal Met and a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
15 kDa
SDS-PAGE
13 kDa, reducing conditions

Product Datasheets

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9287-PO

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9287-PO

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Podocin/NPHS2

Podocin, encoded by the NPHS2 gene, is an approximately 50 kDa membrane protein that plays an important role in podocyte function in the kidney. Loss of Podocin function results in albuminuria, hypercholesterolemia, hypertension, and renal failure (1). Human Podocin consists of a 102 amino acid (aa) cytoplasmic domain, a 21 aa intramembrane segment, and a second 262 aa cytoplasmic domain (2, 3). Alternative splicing generates a short isoform with a 68 aa deletion in the second cytoplasmic domain (4, 5). Within aa 259-383 (the region common to both isoforms), human Podocin shares 90% aa sequence identity with mouse and rat Podocin. Podicin localizes to areas of cell-cell contact between podocytes in the renal glomerulus (2, 6, 7). It associates into oligomers and forms complexes with Nephrin, CAR, ZO-1, and the cation ion channel TRPC6 (6-9). It contributes to podocyte function by regulating the activation of TRPC6 and Nephrin mediated signaling (8, 9). Multiple polymorphisms in NPHS2 are associated with steroid-resistant nephrotic syndrome (2).

References
  1. Mollet, G. et al. (2009) J. Am. Soc. Nephrol. 20:2181.
  2. Boute, N. et al. (2000) Nat. Genet. 24:349.
  3. Roselli, S. et al. (2002) Am. J. Pathol. 160:131.
  4. Volker, L.A. et al. (2013) BMC Nephrol. 14:102.
  5. Relle, M. et al. (2011) Mod. Pathol. 24:1101.
  6. Shono, A. et al. (2007) J. Am. Soc. Nephrol. 18:2525.
  7. Schwarz, K. et al. (2001) J. Clin. Invest. 108:1621.
  8. Huber, T.B. et al. (2001) J. Biol. Chem. 276:41543.
  9. Anderson, M. et al. (2013) Am. J. Physiol. Cell Physiol. 305:C276.
Entrez Gene IDs
7827 (Human); 170484 (Mouse); 170672 (Rat)
Alternate Names
Nephrosis 2; nephrosis 2, idiopathic, steroid-resistant (podocin); NPHS2; PDCN; PDCNSRN1podocin; Podocin; SRN1

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