Recombinant Human Pro-Collagen III alpha 1/COL3A1, CF

R&D Systems | Catalog # 7294-CL

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human Pro-Collagen III alpha 1/COL3A1 (7294-CL)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

NP_000081

Applications

Enzyme Activity
View all Collagen III alpha 1/COL3A1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Collagen III alpha 1/COL3A1 protein
Thr1097-Leu1466, with an N-terminal 6-His tag

Purity

>75%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

40 kDa

SDS-PAGE

41-46 kDa, reducing conditions

Activity

Measured by the cleavage of its C-terminal propeptide by Recombinant Human BMP‑1/PCP (Catalog # 1927-ZN).
>50% of full-length Pro-Collagen III alpha 1 is cleaved by recombinant human BMP-1, as measured under the described conditions.

Formulation, Preparation, and Storage

7294-CL
Formulation Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Collagen III alpha 1/COL3A1

Collagen type III is found in most soft connective tissues, such as skin, lung, and the vascular system, often in association with type I collagen. It is a trimer of
alpha 1(III) chains that are linked by interchain disulfide bonds. Defective collagen type III is a cause of Ehlers-Danlos syndrome types III and IV and arterial aneurisms (1). This recombinant mini pro-alpha 1(III) collagen consists of a triple-helical region of Gly-Xaa-Yaa repeats, a short non-helical region, and the C‑terminal propeptide. The C‑terminal propeptide can be removed by the procollagen C‑proteinase (BMP-1).

References

  1. Kuivaniemi H. et al. (1997) Hum. Mutat. 9:300.

Alternate Names

COL3A1, EDS4A

Entrez Gene IDs

1281 (Human); 12825 (Mouse); 84032 (Rat)

Gene Symbol

COL3A1

UniProt

NP_000081

Additional Collagen III alpha 1/COL3A1 Products

Product Documents for Recombinant Human Pro-Collagen III alpha 1/COL3A1, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Pro-Collagen III alpha 1/COL3A1, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

For research use only

Related Research Areas

Extracellular Matrix Molecules

Citations for Recombinant Human Pro-Collagen III alpha 1/COL3A1, CF

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Protocols

View specific protocols for Recombinant Human Pro-Collagen III alpha 1/COL3A1, CF (7294-CL):

Materials
  • Assay Buffer: 25 mM HEPES, 0.01% Brij, pH 7.5
  • Recombinant Human Pro Collagen III  alpha 1/COL3A1 (rhPro-COL3A1) (Catalog # 7294-CL)
  • Recombinant Human BMP‑1/PCP (rhBMP-1) (Catalog # 1927-ZN)
  • Reducing SDS-PAGE Sample Buffer
  • SDS-PAGE or Western Blot
  1. Dilute rhPro-COL3A1 to 75 μg/mL in Assay Buffer.
  2. Dilute rhBMP-1 to 5 μg/mL in Assay Buffer.
  3. Combine one volume of diluted rhPro-COL3A1 with three volumes of diluted rhBMP-1. For controls, combine one volume of rhPro‑COL3A1 with three volumes of Assay Buffer, as well as three volumes of rhBMP-1 with one volume of Assay Buffer.
  4. Incubate reaction vials and controls at 37 °C for one hour.
  5. After incubation, combine rhPro-COL3A1/rhBMP-1 reaction mixtures and controls with reducing SDS-PAGE gel buffer at a 2:1 (reaction mixture:gel buffer) ratio (v/v). Mix and incubate samples at 95-100 °C for 3-5 minutes to stop reactions.
  6. Load 40 μL (0.5 μg of rhPro-COL3A1) per lane and analyze the cleavage by SDS-PAGE followed by protein staining and/or Western blot.
  7. Activity calculation:
     % Cleavage = [1  -   % full-length rhPro-COL3A1 (reaction) ] x 100%
% full-length rhPro-COL3A1 (control)

Per Lane:

  • rhCOL3A1: 0.5 µg
  • rhBMP-1: 0.1 µg

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