Recombinant Human Properdin Protein, CF

R&D Systems | Catalog # 8216-PR

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human Properdin Protein (8216-PR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

P27918

Applications

Bioactivity
View all Properdin Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Properdin protein
Asp28-Leu469, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp28

Predicted Molecular Mass

50 kDa

SDS-PAGE

57-63 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human Properdin binds to Zymosan with a ED50 of ≤1.25 µg/mL.

Formulation, Preparation, and Storage

8216-PR
Formulation Supplied as a 0.2 μm filtered solution in Sodium Citrate and NaCl.
Reconstitution
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Properdin

Properdin, also known as Complement Factor P, is an approximately 53 kDa glycoprotein that positively regulates the complement alternative pathway (AP) (1, 2). It consists of seven thrombospondin repeat (TSR) domains, referred to as TSR0-TSR6 beginning at the N-terminus (3). Under physiological conditions, Properdin can form cyclic dimers, trimers, and tetramers through head-to-tail interactions of monomeric subunits (4-6). Non-physiological high molecular weight polymers of Properdin can also form during long term storage and freeze/thaw cycles (5, 7). Mature human Properdin shares 79% and 77% amino acid sequence identity with rat and mouse Properdin, respectively. Interestingly, human Properdin has been shown to restore LPS-dependent AP complement activity in Properdin-/- mouse serum (8). Properdin may also act as a pattern-recognition molecule, since it binds glycosaminoglycan (GAG) chains of proteoglycans on apoptotic T cells to promote complement activation and phagocytosis (9). Furthermore, Properdin has been shown to bind certain microbial targets and serve as a platform for C3bBbP assembly and function (10, 11). Underscoring its importance to immunity, Properdin deficiency is associated with significant risk of meningococcal disease in humans (12). It may also be involved in the development of abdominal aortic aneurysm, an inflammatory vascular disease (13).

References

  1. Pillemer, L. et al. (1954) Science 120:279.
  2. Kemper, C. et al. (2010) Annu. Rev. Immunol. 28:131.
  3. Alcorlo, M. et al. (2013) Proc. Natl. Acad. Sci. USA 110:13504.
  4. Smith, C.A. et al. (1984) J. Biol. Chem. 259:4582.
  5. Pangburn, M.K. (1989) J. Immunol. 142:202.
  6. Cortes, C. et al. (2013) Front. Immunol. 3:412.
  7. Farries, T.C. et al. (1987) Biochem. J. 243:507.
  8. Kimura, Y. et al. (2008) Blood 111:732.
  9. Kemper, C. et al. (2008) Proc. Natl. Acad. Sci. USA 105:9023.
  10. Hourcade, D.E. (2006) J. Biol. Chem. 281:2128.
  11. Spitzer, D. et al. (2007) J. Immunol. 179:2600.
  12. Linton, S.M. and B.P. Morgan (1999) Clin. Exp. Immunol. 118:189.
  13. Zhou, H.F. et al. (2012) Proc. Natl. Acad. Sci. USA 109:E415.

Alternate Names

CFP, Complement Factor P, PFC

Entrez Gene IDs

5199 (Human); 18636 (Mouse); 299314 (Rat)

Gene Symbol

CFP

UniProt

P27918

Additional Properdin Products

Product Documents for Recombinant Human Properdin Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Properdin Protein, CF

For research use only

Related Research Areas

Complement Pathway

Citations for Recombinant Human Properdin Protein, CF

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