Recombinant Human Reg4 Protein, CF

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Recombinant Human Reg4 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to support the adhesion and proliferation of HCT‑116 human colorectal carcinoma cells under low serum conditions. The ED50 for this effect is 2-8 µg/mL.
E. coli-derived human Reg4 protein
Asp23-Pro158, with an N-terminal Met
Accession #
N-terminal Sequence
Predicted Molecular Mass
16.0 kDa

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 300 - 500 μg/mL in sterile, deionized water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Reg4

Reg4 (regenerating islet-derived protein 4), also called Reg IV or RELP (Reg-like protein), is a 17 kDa secreted glycoprotein belonging to the regenerating gene (Reg) family within the calcium (C‑type) dependent lectin superfamily, although carbohydrate binding of Reg4 is calcium-independent (1-4). Human Reg4 is synthesized as a 158 amino acid (aa) precursor with a 22 aa signal sequence and a 136 aa mature chain that contains a C‑type lectin-like domain (CTLD) and one potential N-linked glycosylation site. Human Reg4 shares 68% aa sequence identity with mouse and rat, and 77% with canine and porcine Reg4, respectively. Alternate splicing creates a 134 aa isoform that diverges at aa 56, and a 123 aa isoform that diverges at aa 101 (5). Like other members of the regenerating gene family, Reg4 is preferentially expressed in the gastrointestinal (GI) tract (1, 2, 6). Reg4 expression is increased within or near inflammation, dysplasia and metaplasia of the GI epithelium, such as inflammatory bowel disease (Crohn’s disease and ulcerative colitis), colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, and is often increased in the plasma in these conditions (1-3, 6, 7). It is especially associated with neuroendocrine tumors in the GI, as well as some prostate, parathyroid, skin Merkel cell and lung small-cell carcinomas (7-9). Reg4 expression is induced by growth factors and promotes phosphorylation and activation of the EGF R (6, 8, 10). Tumor cells expressing Reg4 are generally more mitogenic, metastatic and resistant to apoptosis (11-13).

  1. Hartupee, J.C. et al. (2001) Biochim. Biophys. Acta 1518:287.
  2. Zhang, Y-W. et al. (2003) World J. Gastroenterol. 9:2635.
  3. Kamarainen, M. et al. (2003) Am. J. Pathol. 163:11.
  4. Ho, M.R. et al. (2010) J. Mol. Biol. 402:682.
  5. Protein accession Q9BYZ8-1, Q9BYZ8-2, EAW56711.
  6. Nanakin, A. et al. (2007) Lab. Invest. 87:304.
  7. Oue, N. et al. (2005) J. Pathol. 207:185.
  8. Ohara, S. et al. (2008) Cancer Sci. 99:1570.
  9. Heiskala, K. et al. (2010) Histol. Histopathol. 25:63.
  10. Bishnupuri K.S. et al. (2006) Gastroenterology 130:137.
  11. Rafa, L. et al. (2010) Int. J. Oncol. 36:689.
  12. Bishnupuri K.S. et al. (2010) Gastroenterology 138:616.
  13. Guo, Y. et al. (2010) Cancer Genet. Cytogenet. 199:38.
Long Name
Regenerating Islet-derived 4
Entrez Gene IDs
83998 (Human); 67709 (Mouse); 445583 (Rat)
Alternate Names
Gastrointestinal secretory protein; GISP; GISPREG-4; Reg IV; Reg4; regenerating gene type IV; regenerating islet-derived family, member 4; regenerating islet-derived protein 4; Regenerating islet-derived protein IV; REG-IV; RELP; RELPREG-like protein


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Recombinant Human Reg4 Protein, CF
By Anonymous on 10/04/2019
Application: Immunoassay Standard

We used this protein for a sandwich ELISA format in combination with pAb (AF1379) and mAb (MAB1379). This combination worked very well and detected human Reg 4 in serum and plasma samples.