Recombinant Human Reg4 Protein, CF

R&D Systems | Catalog # 1379-RG

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human Reg4 Protein (1379-RG)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

Q9BYZ8

Applications

Bioactivity
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Product Specifications

Source

E. coli-derived human Reg4 protein
Asp23-Pro158, with an N-terminal Met

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

16.0 kDa

Activity

Measured by the ability of the immobilized protein to support the adhesion and proliferation of HCT‑116 human colorectal carcinoma cells under low serum conditions.
The ED50 for this effect is 2-8 µg/mL.

Reviewed Applications

Read 1 review rated 5 using 1379-RG in the following applications:

Formulation, Preparation, and Storage

1379-RG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 300 - 500 μg/mL in sterile, deionized water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Reg4

Reg4 (regenerating islet-derived protein 4), also called Reg IV or RELP (Reg-like protein), is a 17 kDa secreted glycoprotein belonging to the regenerating gene (Reg) family within the calcium (C‑type) dependent lectin superfamily, although carbohydrate binding of Reg4 is calcium-independent (1-4). Human Reg4 is synthesized as a 158 amino acid (aa) precursor with a 22 aa signal sequence and a 136 aa mature chain that contains a C‑type lectin-like domain (CTLD) and one potential N-linked glycosylation site. Human Reg4 shares 68% aa sequence identity with mouse and rat, and 77% with canine and porcine Reg4, respectively. Alternate splicing creates a 134 aa isoform that diverges at aa 56, and a 123 aa isoform that diverges at aa 101 (5). Like other members of the regenerating gene family, Reg4 is preferentially expressed in the gastrointestinal (GI) tract (1, 2, 6). Reg4 expression is increased within or near inflammation, dysplasia and metaplasia of the GI epithelium, such as inflammatory bowel disease (Crohn’s disease and ulcerative colitis), colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, and is often increased in the plasma in these conditions (1-3, 6, 7). It is especially associated with neuroendocrine tumors in the GI, as well as some prostate, parathyroid, skin Merkel cell and lung small-cell carcinomas (7-9). Reg4 expression is induced by growth factors and promotes phosphorylation and activation of the EGF R (6, 8, 10). Tumor cells expressing Reg4 are generally more mitogenic, metastatic and resistant to apoptosis (11-13).

References

  1. Hartupee, J.C. et al. (2001) Biochim. Biophys. Acta 1518:287.
  2. Zhang, Y-W. et al. (2003) World J. Gastroenterol. 9:2635.
  3. Kamarainen, M. et al. (2003) Am. J. Pathol. 163:11.
  4. Ho, M.R. et al. (2010) J. Mol. Biol. 402:682.
  5. Protein accession Q9BYZ8-1, Q9BYZ8-2, EAW56711.
  6. Nanakin, A. et al. (2007) Lab. Invest. 87:304.
  7. Oue, N. et al. (2005) J. Pathol. 207:185.
  8. Ohara, S. et al. (2008) Cancer Sci. 99:1570.
  9. Heiskala, K. et al. (2010) Histol. Histopathol. 25:63.
  10. Bishnupuri K.S. et al. (2006) Gastroenterology 130:137.
  11. Rafa, L. et al. (2010) Int. J. Oncol. 36:689.
  12. Bishnupuri K.S. et al. (2010) Gastroenterology 138:616.
  13. Guo, Y. et al. (2010) Cancer Genet. Cytogenet. 199:38.

Long Name

Regenerating Islet-derived 4

Alternate Names

GISP, RELP

Entrez Gene IDs

83998 (Human); 67709 (Mouse); 445583 (Rat)

Gene Symbol

REG4

UniProt

Q9BYZ8

Additional Reg4 Products

Product Documents for Recombinant Human Reg4 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human Reg4 Protein, CF

For research use only

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  • Recombinant Human Reg4 Protein, CF
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 10/04/2019
    We used this protein for a sandwich ELISA format in combination with pAb (AF1379) and mAb (MAB1379). This combination worked very well and detected human Reg 4 in serum and plasma samples.
    Recombinant Human Reg4 Protein, CF 1379-RG

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