Recombinant Human SALM3 Protein, CF

Catalog # Availability Size / Price Qty
5445-SA-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human SALM3 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells. When 5 x 104 cells/well are added to Recombinant Human SALM3/LRFN4 coated plates (2.5 μg/mL, 100 μL/well). approximately 50%-70% will adhere after 30 minutes at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived human SALM3/LRFN4 protein
Cys17-Leu518 with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Cys17
Predicted Molecular Mass
54.2 kDa
SDS-PAGE
70-90 kDa, reducing conditions

Product Datasheets

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5445-SA

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5445-SA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: SALM3/LRFN4

Synaptic adhesion-like molecule 3 (SALM3; also leucine-rich repeat and fibronectin type-III domain-containing protein 4 (Lrfn4) is an approximately 90 kDa member of the Lrfn family of type I transmembrane glycoproteins (1).  Human SALM3 is synthesized as a 635 amino acid (aa) precursor that contains a 16 aa signal sequence, a 502 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 96 aa cytoplasmic region.  The ECD consists of seven leucine-rich repeats (LRR), an IgC2‑like domain, and a fibronectin type-III domain, tandemly aligned in that order (1-2).  In addition, there are six potential sites for N-linked glycosylation. The C‑terminal region contains an intracellular PDZ binding domain, which is conserved among SALMs 1-3, but is absent in SALMs 4 and 5 (3). Mature human SALM3 shares 97% aa sequence identity with mature mouse SALM3.  Northern blot analysis showed that in mice, SALM3 is strongly expressed in the adult brain and is also present in the adult testis (1). It is distributed throughout the neuron, including the growth cone (3).  In the developing mouse embryo, a temporal expression profile blot revealed a general increment of expression around E10.5, with weak expression detected before E10.5 (1). SALM3, like the other SALMs, promotes neurite outgrowth (3).  Specifically, the SALMs modify total outgrowth and neurite branching (3).  SALM3 may also be involved in synapse formation, synaptic maintenance, and other cellular interactions (3).

References
  1. Morimura, N. et al. (2006) Gene 380:72.
  2. Wang, C.-Y. et al. (2006) J. Neurosci. 26:2174.
  3. Wang, P.Y. et al. (2008) Mol. Cell. Neurosci. 39:83.
Long Name
Synaptic Adhesion-like Molecule 3/Leucine-rich Repeat and Fibronectin Type III Domain Containing 4
Entrez Gene IDs
78999 (Human); 225875 (Mouse); 688721 (Rat)
Alternate Names
FIGLER6; LRFN4; SALM3; SALM3.

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