Recombinant Human SCUBE3 Protein, CF

Catalog # Availability Size / Price Qty
7730-SC-050
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Recombinant Human SCUBE3 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is typically 0.25-1.25 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human SCUBE3 protein
Ala21-Lys993, with an N-terminal HA-tag (YPYDVPDYA)
Accession #
N-terminal Sequence
Analysis
Tyr & Arg538
Predicted Molecular Mass
108.3 kDa (Monomer)
SDS-PAGE
110-130 kDa & 60-70 kDa, reducing conditions  

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7730-SC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: SCUBE3

SCUBE3 (signal peptide, CUB and EGF-like domain-containing protein 3) is a member of the SCUBE family of secreted glycoproteins (1). Like other SCUBE proteins, the 993 amino acid (aa) human SCUBE3 precursor contains a signal peptide (aa 1‑20), nine EGF‑like motifs (aa 29‑398), a linker region with multiple N‑glycosylation sites (aa 399‑803) and a C‑terminal CUB domain (aa 804‑916). Cysteines within the EGF‑like and linker regions are mainly conserved (1). The 130 kDa, mature, full‑length form of human SCUBE3 shares 96%, 93%, 96%, 95%, 95% and 94% aa sequence identity with mouse, rat, equine, bovine, canine and porcine SCUBE3, respectively (1). It also shares 66% and 58% aa identity with human SCUBE1 and SCUBE2, respectively. Cleavage by proteases such as MMP‑2 and MMP‑9 produces a CUB domain-containing, 55 kDa fragment that, like full‑length SCUBE3, binds TGF-beta RII (1, 2). SCUBE proteins form homo-oligomers and hetero‑oligomers, and are predicted to affect TGF‑ beta and Hedgehog signaling (2‑5). SCUBE3 is most highly expressed in osteoblasts (1, 3, 6). It is also found in umbilical vein endothelial cells with SCUBE1 and SCUBE2, and in cardiac ventricular myocytes (1, 3, 6). Transgenic over-expression of SCUBE3 can cause progressive cardiac hypertrophy (3). SCUBE3 and its active fragment are frequently over‑expressed in lung cancer tumor tissues, and can promote epithelial to mesenchymal transition by enhancing TGF-beta signaling pathways (2).

References
  1. Wu, B.T. et al. (2004) J. Biol. Chem. 279:37485.
  2. Wu, Y.Y. et al. (2011) Oncogene 30:3682.
  3. Yang, H.Y. et al. (2007) Cardiovasc. Res. 75:139.
  4. Johnson, J.L. et al. (2012) Dev. Biol. 368:193.
  5. Creanga, A. et al. (2012) Genes Dev. 26:1312.
  6. Haworth, K. et al. (2007) Gene Expr. Patterns 7:630.
Entrez Gene IDs
222663 (Human); 268935 (Mouse); 294297 (Rat)
Alternate Names
CEGF3; CUB and EGF containing protein 3; CUB domain and EGF-like repeat containing 3; DKFZp686B09105; DKFZp686B1223; DKFZp686D20108; FLJ34743; SCUBE3; signal peptide, CUB and EGF-like domain containing protein 3; signal peptide, CUB and EGF-like domain-containing protein 3; signal peptide, CUB domain, EGF-like 3

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