Recombinant Human Semaphorin 3D Fc Chimera Protein, CF

R&D Systems | Catalog # 9674-S3

R&D Systems
Discontinued Product
9674-S3 has been discontinued. View all Semaphorin 3D products.

Key Product Details

Source

CHO

Accession Number

O95025

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all Semaphorin 3D Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Semaphorin 3D protein
Human Semaphorin 3D
(Thr37-His736 (Arg571Ala) & (Arg572Ala))
Accession # O95025		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr37

Predicted Molecular Mass

107 kDa

SDS-PAGE

98-113 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Semaphorin 3D Fc Chimera is coated at 1 µg/mL, 100 μL/well, Recombinant Rat Neuropilin‑1 Fc Chimera (Catalog # 566-N1) binds with an ED50 of 0.5-2.5 μg/mL.

Formulation, Preparation, and Storage

9674-S3
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Semaphorin 3D

Semaphorin 3D (Sema3D) is a member of the semaphorin family that plays a role in neuronal and vascular development (1, 2). Class 3 semaphorins are secreted molecules, and contain a common domain structure. The human Sema3D domains consist of a 488 amino acid (aa) Sema domain, a 53 aa plexin-semaphorin-integrin (PSI) domain, an 89 aa Ig-like C2-type domain, and a 35 aa basic domain which is proteolytically processed (3, 4). Within these domains, human Sema3D shares 94% aa sequence identity with mouse and rat Sema3D. Sema3D induces the collapse and paralysis of neuronal growth cones and promotes fasciculation of axons (5, 6). Sema3D also inhibits endothelial cell motility and tubulogenesis through cytoskeletal reorganization (2). Binding to Neuropilin-I is required for Sema3D signaling (7). ErbB2 was shown to be a potential Sema3D co-receptor, which is phosphorylated in response to Sema3D in HUVEC cells (7). Sema3D inhibition of vascular development facilitates inhibition of glioblastoma growth in the cortex of the brain (1).

References

  1. Sabag, A. et al. (2012) PLoS One. 7:e42912.
  2. Aghajanian, H. et al. (2014) J. Biol. Chem. 289:17971.
  3. Takahashi, K. et al. (2009) Biochimica et Biophysica Acta. 1790:395.
  4. Gu, C. and E. Giraudo. (2013) Exp. Cell Res. 319:1306.
  5. Kuhn, T.B. et al. (2000) J. Neurobiology. 44:126.
  6. Wolman, M.A. et al. (2007) J. Neurobiology. 27:9653.
  7. Aghajanian, H. et al. (2015) Nature Commun. 7:12038.

Alternate Names

coll-2, Collapsin 2, Sema-Z2, SEMA3D, Semaphorin-3D

Entrez Gene IDs

223117 (Human); 108151 (Mouse); 246262 (Rat)

Gene Symbol

SEMA3D

UniProt

O95025

Additional Semaphorin 3D Products

Product Documents for Recombinant Human Semaphorin 3D Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Semaphorin 3D Fc Chimera Protein, CF

For research use only

Related Research Areas

Proteoglycans

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