Recombinant Human sFRP-1 Protein, CF

Catalog # Availability Size / Price Qty
5396-SF-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (15)
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Recombinant Human sFRP-1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. Ko, L. et al. (2002) Exp. Cell Res. 280:280. The ED50 for this effect is 0.15‑0.75 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human sFRP-1 protein
Ser32-Lys314
Accession #
N-terminal Sequence
Analysis
Ser32, Phe39 (minor) & Asp42 (minor)
Predicted Molecular Mass
32.6 kDa
SDS-PAGE
33-40 kDa, reducing conditions

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5396-SF

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5396-SF

Formulation Lyophilized from a 0.2 μm filtered solution in Phosphate and NaCl.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: sFRP-1

Secreted Frizzled Related Protein-1 (sFRP-1), also known as SARP-2, FRP, and FrzA, belongs to a family of Wnt-binding proteins with homology to the ligand-binding domain of the Frizzled transmembrane Wnt receptors. The sFRP proteins are approximately 30-35 kDa in size and contain an N-terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1-3). Mature human sFRP-1 shares 97% and 96% amino acid sequence identity with mouse and rat sFRP-1, respectively. It binds to Wnt-1, -2, -3a, and -4 and prevents their activation of canonical beta-catenin signaling (4-7). sFRP-1 is up-regulated in the ischemic heart where it limits inflammation and the severity of tissue damage following myocardial infarction (8-10). During angiogenesis it induces endothelial cell migration, tube formation, and vessel formation (11). sFRP-1 is expressed by bone marrow stromal cells and osteoclasts (12). It binds to TRANCE/RANK Ligand, inhibits osteoclast and osteoblast formation and survival, and inhibits the formation of trabecular bone (13, 14). sFRP-1 additionally restricts kidney tubule formation (7), the inflammatory activation of leukocytes (10), early B lymphoid progression (12), and vitreous fluid outflow in the eye (15).

References
  1. Cruciat, C.M. and C. Niehrs (2013) Cold Spring Harb. Perspect. Biol. 5:e015081.
  2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
  3. Finch, P.W. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6770.
  4. Bafico, A. et al. (1999) J. Biol. Chem. 274:16180.
  5. Dennis, S. et al. (1999) J. Cell Sci. 112:3815.
  6. Wawrzak, D. et al. (2007) Biochem. Biophys. Res. Commun. 357:1119.
  7. Yoshino, K. et al. (2001) Mech. Dev. 102:45.
  8. Barandon, L. et al. (2002) Circulation 108:2282.
  9. Sklepkiewicz, P. et al. (2015) Circ. Heart Fail. 8:362.
  10. Barandon, L. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:e80.
  11. Dufourcq, P. et al. (2002) Circ. Res. 106:3097.
  12. Yokota, T. et al. (2008) J. Immunol. 181:6061.
  13. Hausler, K.D. et al. (2004) J. Bone Miner. Res. 19:1873.
  14. Bodine, P.V.N. et al. (2004) Mol. Endocrinol. 18:1222.
  15. Wang, W.-H. et al. (2008) J. Clin. Invest. 118:1056.
Long Name
Secreted Frizzled Related Protein 1
Entrez Gene IDs
6422 (Human); 20377 (Mouse); 84402 (Rat)
Alternate Names
FRP1; FRP-1FrzA; FRPSARP-2; FrzA; SARP-2; SARP2sFRP-1; Secreted apoptosis-related protein 2; secreted frizzled-related protein 1; sFRP1; sFRP-1

Citations for Recombinant Human sFRP-1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Extrinsic Regulators of mRNA Translation in Developing Brain: Story of WNTs
    Authors: Y Park, M Lofton, D Li, MR Rasin
    Cells, 2021;10(2):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Serum circulating proteins from pediatric dilated cardiomyopathy patients cause pathologic remodeling and cardiomyocyte stiffness
    Authors: DA Jeffrey, J Pires Da S, AM Garcia, X Jiang, A Karimpour-, LS Toni, T Lanzicher, B Peña, CA Miyano, K Nunley, A Korst, O Sbaizero, MR Taylor, SD Miyamoto, BL Stauffer, CC Sucharov
    JCI Insight, 2021;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Wnt Signaling Mediates LTP-Dependent Spine Plasticity and AMPAR Localization through Frizzled-7 Receptors
    Authors: F McLeod, A Bossio, A Marzo, L Ciani, S Sibilla, S Hannan, GA Wilson, E Palomer, TG Smart, A Gibb, PC Salinas
    Cell Rep, 2018;23(4):1060-1071.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Identifying novel strategies for treating human hair loss disorders: Cyclosporine A suppresses the Wnt inhibitor, SFRP1, in the dermal papilla of human scalp hair follicles
    Authors: NJ Hawkshaw, JA Hardman, IS Haslam, A Shahmalak, A Gilhar, X Lim, R Paus
    PLoS Biol., 2018;16(5):e2003705.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Rab37 mediates exocytosis of secreted frizzled-related protein 1 to inhibit Wnt signaling and thus suppress lung cancer stemness
    Authors: SH Cho, IY Kuo, PF Lu, HT Tzeng, WW Lai, WC Su, YC Wang
    Cell Death Dis, 2018;9(9):868.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. A Wnt5 Activity Asymmetry and Intercellular Signaling via PCP Proteins Polarize Node Cells for Left-Right Symmetry Breaking
    Authors: K Minegishi, M Hashimoto, R Ajima, K Takaoka, K Shinohara, Y Ikawa, H Nishimura, AP McMahon, K Willert, Y Okada, H Sasaki, D Shi, T Fujimori, T Ohtsuka, Y Igarashi, TP Yamaguchi, A Shimono, H Shiratori, H Hamada
    Dev. Cell, 2017;40(5):439-452.e4.
    Species: Mouse
    Sample Types: Whole Embryo
    Applications: Bioassay
  7. BMP-induced reprograming of the retina into RPE requires WNT signalling in the developing chick optic cup
    Authors: J Steinfeld, I Steinfeld, A Bausch, N Coronato, ML Hampel, H Depner, PG Layer, A Vogel-Höpk
    Biol Open, 2017;0(0):.
    Species: Chicken
    Sample Types: In Vivo
    Applications: In Vivo
  8. Age-related increase in Wnt inhibitor causes a senescence-like phenotype in human cardiac stem cells
    Authors: T Nakamura, T Hosoyama, J Murakami, M Samura, K Ueno, H Kurazumi, R Suzuki, A Mikamo, K Hamano
    Biochem. Biophys. Res. Commun., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Endothelial cells regulate ?-catenin activity in adrenocortical cells via secretion of basic fibroblast growth factor
    Authors: Holger S Willenberg
    Mol. Cell. Endocrinol., 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Adipose- and muscle-derived Wnts trigger pancreatic ?-cell adaptation to systemic insulin resistance
    Sci Rep, 2016;6(0):31553.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  11. WNT7B mediates autocrine Wnt/beta-catenin signaling and anchorage-independent growth in pancreatic adenocarcinoma.
    Authors: Arensman M, Kovochich A, Kulikauskas R, Lay A, Yang P, Li X, Donahue T, Major M, Moon R, Chien A, Dawson D
    Oncogene, 2014;33(7):899-908.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Wnt-frizzled signaling is part of an FGF-induced cascade that promotes lens fiber differentiation.
    Authors: Dawes L, Sugiyama Y, Tanedo A, Lovicu F, McAvoy J
    Invest Ophthalmol Vis Sci, 2013;54(3):1582-90.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  13. Wnt5a regulates midbrain dopaminergic axon growth and guidance.
    Authors: Blakely BD, Bye CR, Fernando CV
    PLoS ONE, 2011;6(3):e18373.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Coordination of epithelial branching and salivary gland lumen formation by Wnt and FGF signals.
    Authors: Patel N, Sharpe PT, Miletich I
    Dev. Biol., 2011;358(1):156-67.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Paracrine and autocrine signals induce and maintain mesenchymal and stem cell states in the breast.
    Authors: Scheel C, Eaton E, Li S, Chaffer C, Reinhardt F, Kah K, Bell G, Guo W, Rubin J, Richardson A, Weinberg R
    Cell, 2011;145(6):926-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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