Recombinant Human Siglec-11 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
3258-SL-050
Product Details
Citations (1)
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Recombinant Human Siglec-11 Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind biotinylated (Neu5Aca2-8)2-Polyacrylamide in functional ELISA. Angata, T. et al. (2002) J. Biol. Chem. 277(27):24466. Recombinant Human Siglec‑11 Fc Chimera immobilized at 2.5 µg/mL (100 µL/well), on a Goat Anti-Human IgG Fc Antibody
(Catalog # G-102-C) coated plate can bind biotinylated (Neu5Ac alpha 2-8)2-Polyacrylamide with a linear range of 0.03-2 µg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Siglec-11 protein
Human Siglec-11
Asn17-His543
(Glu84Ala & Lys145Gln)
Accession # AAK72907
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Asn17
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
84.4 kDa (monomer)
SDS-PAGE
110-135 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3258-SL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Siglec-11

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by anN-terminal Ig-like V-set domain which mediates sialic acid binding, followed by a varying numbers of Ig-like C2-set domains. Siglecs-3 and 5 - 13 constitute the CD33/Siglec-3 related group, which are defined by their sequence homology and differential expression in the hematopoietic system (1-3). Mature human Siglec-11 consists of a 534 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 114 aa cytoplasmic domain. The ECD contains one Ig-like V-set domain, and three Ig-like C2-set domains. The cytoplasmic domain contains two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (4). A splice variant of Siglec-11 has a deletion of nearly 100 aa in the extracellular juxtamembrane region. Among siglecs, the ECD of Siglec-11 is most closely related to that of Siglec-10 (82% aa sequence identity). The cytoplasmic domains of these proteins are only 20% identical. Siglec-11 is closely related to the pseudogenes Siglec-14 and Siglec-16 (4, 5). Human Siglec-11 shares 90%-96% aa sequence identity with Siglec-11 from great apes. Rodent orthologs of Siglec-11 have not been identified. In human, Siglec-11 is expressed in tissue macrophages, brain microglia, and inflammatory site monocytes (4). Strong microglial expression is specific to humans, as it is less prominent or absent in chimpanzees and orangutans (5). Siglec-11 forms 180 kDa disulfide-linked dimers. It shows a strong binding preference for sialic acid in alpha 2-8 linkage which is unusual for siglecs (4). A conserved arginine in the Ig-like V-set domain only partially contributes to Siglec-11 ligand recognition, in contrast to its being required in other siglecs (4). Tyrosine phosphorylation of the cytoplasmic region of Siglec-11 promotes association with the phosphatases SHP-1 and SHP-2 (4).

References
  1. Varki, A. and T. Angata (2006) Glycobiology 16:1R.
  2. Crocker, P.R. (2005) Curr. Opin. Pharmacol. 5:431.
  3. Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609.
  4. Angata, T. et al. (2002) J. Biol. Chem. 277:24466.
  5. Hayakawa, T. et al. (2005) Science 309:1693.
Long Name
Sialic Acid Binding Ig-like Lectin 11
Entrez Gene IDs
114132 (Human)
Alternate Names
sialic acid binding Ig-like lectin 11; sialic acid-binding Ig-like lectin 11; Sialic acid-binding lectin 11; Siglec11; Siglec-11

Citation for Recombinant Human Siglec-11 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Broad and direct interaction between TLR and Siglec families of pattern recognition receptors and its regulation by Neu1.
    Authors: Chen, Guo-Yun, Brown, Nicholas, Wu, Wei, Khedri, Zahra, Yu, Hai, Chen, Xi, van de Vlekkert, Diantha, D'Azzo, Alessand, Zheng, Pan, Liu, Yang
    Elife, 2014;3(0):e04066.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay

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