Recombinant Human SIRP beta 2 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9998-SB-050
Recombinant Human SIRP beta 2 Fc Chimera Protein, CF Binding Activity
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Recombinant Human SIRP beta 2 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human SIRP beta 2 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human CD300B/LIMR5 Fc Chimera binds with an ED50 of 2-10 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human SIRP beta 2 protein
          
Human SIRP beta 2
(Gln33-Gly287)
Accession # Q5JXA9
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
No results obtained. Gln33 inferred from enzymatic pyroglutamate treatment revealing Ser34.
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
55 kDa
SDS-PAGE
77-85 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9998-SB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human SIRP beta 2 Fc Chimera (Catalog #9998-SB) is immobilized at 1 µg/mL, Biotinylated Recombinant CD300B/LMIR-5 Fc Chimera binds with an ED50 of 2‑10 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human SIRP beta 2 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 77-85 kDa and 150-170 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SIRP beta 2

Signal-regulatory protein beta-2(SIRP-beta-2), is a ~37 kDa monomeric single pass type I membrane glycoprotein. It belongs to the SIRP/SHPS (CD172) family of the immunoglobulin (Ig) superfamily (1). The SIRP family are paired receptors that have similar extracellular domains but differing C-terminal domains and functions (1). SIRP-beta-2 contains an N-terminal signal peptide (aa1-32), two extracellular Ig-like domains: a V-type 1 (aa 33-143) and a V-type 2 (aa 157-258) containing three potential N-linked glycosylation sites, a helical transmembrane domain (aa 288-308), and a cytoplasmic domain (aa 309-342) (1). A positively charged residue within the transmembrane domain, in analogy to SIRP-beta-1, is implicated to mediate interaction with the adaptor DAP12 protein, which contains immunoreceptor tyrosine-based activation motifs (ITAMs) (2). Proteins in the SIRP family are typically expressed in immune cells, especially in the myeloid lineages (3). Based on expression patterns, SIRPs are thought to have roles in immune regulation (4). SIRP family members role in innate immunity and host defense has potential significance as a therapeutic target in cancer and inflammation (5, 6). There are currently no known mouse or rat homologs for this protein.

References
  1. van Beek, E.M. et al. (2005) J. Immunol. 175:7781.
  2. Liu, Y. et al. (2005) Journal of Biological Chemistry. 280:36132.
  3. Matozaki, T. et al. (2009) Trends in Cell Biology. 19:72.
  4. Barclay A.N. et al. (2006) Nat Rev Immunol. 6:457.
  5. Barclay A.N. et al. (2014) Annu Rev Immunol. 32:25.
  6. Veillette A. (2018) Trends Immunol. 39:173.
Long Name
Signal-regulatory Protein beta 2
Entrez Gene IDs
284759 (Human)
Alternate Names
dJ776F14.2; Protein Tyrosine Phosphatase Non-Receptor Type Substrate 1-Like 3; Protein Tyrosine Phosphatase Non-Receptor Type Substrate Protein; Protein Tyrosine Phosphatase, Non-Receptor Type 1-Like; Protein Tyrosine Phosphatase, Non-Receptor Type Substrate 1-Like 3; PTPN1L; PTPNS1L3; Signal-Regulatory Protein Beta 2; Signal-Regulatory Protein Beta-2; SIRP beta 2; SIRP-beta-2; SIRPG

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