Recombinant Human SKP1/FBXO7/CUL1/RBX1 Complex Protein, CF

Catalog #: E3-526 Datasheet / COA / SDS

Discontinued Product

E3-526 has been discontinued.
View all SKP1/FBX07/CUL1/RBX1 Complex products.
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Product Details

Recombinant Human SKP1/FBXO7/CUL1/RBX1 Complex Protein, CF Summary

Product Specifications

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Typical protein concentration for use in vitro will depend on experimental conditions.  We recommend an initial starting concentration of 10-50 nM for in vitro ubiquitination reactions.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human SKP1/FBX07/CUL1/RBX1 Complex protein
Predicted Molecular Mass
21 kDa (SKP1), 63kDa (FBXO7), 96 kDa (CUL1), 12 kDa (RBX1)

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 60 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Background: SKP1/FBX07/CUL1/RBX1 Complex

SKP1 (S-phase kinase-associated protein 1) is an essential component of SCF (SKP1-CUL1-F-box protein) E3 Ubiquitin ligases that mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.  SKP1 is the adapter protein that links Cullin-1 to various F-box proteins (such as FBXO7) that serve as the substrate recognition components of SCF ligases.  FBXO7 (aka FBX7 or PARK15) plays a role in mitophagy by helping target Parkin (PARK2) to depolarized mitochondria, thereby inducing the ubiquitination of TOMM20 and other mitochondrial proteins.  Evidence from a Drosophila Parkin-/- neurodegeneration model indicates that FBXO7 can substitute for Parkin-mediated ubiquitination of mitochondrial substrates in some settings.  This recombinant protein complex consists of His-tag SKP1, FLAG-tag FBXO7, His-tag CUL1 and untagged RBX1.

  1. Burchell V.S., et al. (2013) Nat. Neurosci.  16: 1257
  2. Chang Y.F. et al. (2006) Biochem. Biophys. Res. Comm.  342: 1022
  3. Hsu J.M., et al. (2004) J. Biol. Chem.  279: 32592
  4. Randle S.J. & Laman H. (2017) Curr. Protein Pept. Sci.  18: 715
  5. Stott S.R.W. et al. (2019) J. Pathol.  249: 241
Alternate Names
SKP1/FBX07/CUL1/RBX1 Complex


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