Recombinant Human SKP1/FBXO7/CUL1/RBX1 Complex Protein, CF Summary
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: SKP1/FBX07/CUL1/RBX1 Complex
(S-phase kinase-associated protein 1) is an essential component of SCF
(SKP1-CUL1-F-box protein) E3 Ubiquitin ligases that mediate the
ubiquitination of proteins involved in cell cycle progression, signal
transduction and transcription. SKP1 is the adapter protein that links
Cullin-1 to various F-box proteins (such as FBXO7) that serve as the
substrate recognition components of SCF ligases. FBXO7 (aka FBX7 or
PARK15) plays a role in mitophagy by helping target Parkin (PARK2) to
depolarized mitochondria, thereby inducing the ubiquitination of TOMM20
and other mitochondrial proteins. Evidence from a Drosophila Parkin-/-
neurodegeneration model indicates that FBXO7 can substitute for
Parkin-mediated ubiquitination of mitochondrial substrates in some
settings. This recombinant protein complex consists of His-tag SKP1, FLAG-tag FBXO7, His-tag CUL1 and untagged RBX1.
- Burchell V.S., et al. (2013) Nat. Neurosci. 16: 1257
- Chang Y.F. et al. (2006) Biochem. Biophys. Res. Comm. 342: 1022
- Hsu J.M., et al. (2004) J. Biol. Chem. 279: 32592
- Randle S.J. & Laman H. (2017) Curr. Protein Pept. Sci. 18: 715
- Stott S.R.W. et al. (2019) J. Pathol. 249: 241
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