Recombinant Human SKP1/FBXO7/CUL1/RBX1 Complex Protein, CF

R&D Systems | Catalog # E3-526

R&D Systems
Discontinued Product
E3-526 has been discontinued. View all SKP1/FBX07/CUL1/RBX1 Complex products.

Key Product Details

Source

Sf 21 (baculovirus)

Accession Number

P63208, Q9Y3I1, Q13616, P62877

Applications

Bioactivity
View all SKP1/FBX07/CUL1/RBX1 Complex Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human SKP1/FBX07/CUL1/RBX1 Complex protein

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

21 kDa (SKP1), 63kDa (FBXO7), 96 kDa (CUL1), 12 kDa (RBX1)

Activity

Typical protein concentration for use in vitro will depend on experimental conditions.  We recommend an initial starting concentration of 10-50 nM for in vitro ubiquitination reactions.

Formulation, Preparation, and Storage

E3-526
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 60 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: SKP1/FBX07/CUL1/RBX1 Complex

SKP1 (S-phase kinase-associated protein 1) is an essential component of SCF (SKP1-CUL1-F-box protein) E3 Ubiquitin ligases that mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.  SKP1 is the adapter protein that links Cullin-1 to various F-box proteins (such as FBXO7) that serve as the substrate recognition components of SCF ligases.  FBXO7 (aka FBX7 or PARK15) plays a role in mitophagy by helping target Parkin (PARK2) to depolarized mitochondria, thereby inducing the ubiquitination of TOMM20 and other mitochondrial proteins.  Evidence from a Drosophila Parkin-/- neurodegeneration model indicates that FBXO7 can substitute for Parkin-mediated ubiquitination of mitochondrial substrates in some settings.  This recombinant protein complex consists of His-tag SKP1, FLAG-tag FBXO7, His-tag CUL1 and untagged RBX1.

References

  1. Burchell V.S., et al. (2013) Nat. Neurosci.  16: 1257
  2. Chang Y.F. et al. (2006) Biochem. Biophys. Res. Comm.  342: 1022
  3. Hsu J.M., et al. (2004) J. Biol. Chem.  279: 32592
  4. Randle S.J. & Laman H. (2017) Curr. Protein Pept. Sci.  18: 715
  5. Stott S.R.W. et al. (2019) J. Pathol.  249: 241

UniProt

P63208, Q9Y3I1, Q13616, P62877

Product Documents for Recombinant Human SKP1/FBXO7/CUL1/RBX1 Complex Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human SKP1/FBXO7/CUL1/RBX1 Complex Protein, CF

For research use only

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Citations for Recombinant Human SKP1/FBXO7/CUL1/RBX1 Complex Protein, CF

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