Recombinant Human SKP1/SKP2 Complex Protein, CF

R&D Systems | Catalog # E3-521

R&D Systems
Discontinued Product
E3-521 has been discontinued. View all SKP1/SKP2 Complex products.

Key Product Details

Source

Sf 21 (baculovirus)

Applications

Bioactivity
View all SKP1/SKP2 Complex Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human SKP1/SKP2 Complex protein

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

19 kDa (SKP1), 48 kDa (SKP2)

Activity

Typical protein concentration for use in vitro will depend on experimental conditions.

Formulation, Preparation, and Storage

E3-521
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 36 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: SKP1/SKP2 Complex

SKP1 (S-phase kinase-associated protein 1) is an essential component of SCF (SKP1-CUL1-F-box protein) E3 Ubiquitin ligases that mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.  SKP1 is the adapter protein that links Cullin-1 to various F-box proteins (such as SKP2) that serve as the substrate recognition components of SCF ligases.  SKP2 targets include proteins involved in cell cycle progression and signal transduction.  SKP2 recognizes phosphorylated CDKN1B/p27Kip and is involved in G1-S cell cycle transition in a CKS1-dependent manner.

References

  1. Goldenberg S.J. et al. (2004) Cell  119: 517
  2. Hristova V.A. et al. (2012) Mol. Cell  47: 331
  3. Tedesco D. et al. (2002) Genes & Dev.  doi:10.1101/gad.1011202
  4. Wang W. et al. (2005) Biochemistry  doi.org/10.1021/bi051071j
  5. Wu G. et al. (2003) Mol. Cell  11: 1445

Long Name

S-phase Kinase Associated Protein 1/S-phase Kinase Associated Protein 2 Complex

Entrez Gene IDs

6500 (Human)

Gene Symbol

SKP1

Product Documents for Recombinant Human SKP1/SKP2 Complex Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human SKP1/SKP2 Complex Protein, CF

For research use only

Related Research Areas

Deubiquitinating Enzymes (DUBs)

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