Recombinant Human SKP1/SKP2 Complex Protein, CF Summary
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: SKP1/SKP2 Complex
SKP1 (S-phase kinase-associated protein 1) is an essential component of SCF (SKP1-CUL1-F-box protein) E3 Ubiquitin ligases that mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SKP1 is the adapter protein that links Cullin-1 to various F-box proteins (such as SKP2) that serve as the substrate recognition components of SCF ligases. SKP2 targets include proteins involved in cell cycle progression and signal transduction. SKP2 recognizes phosphorylated CDKN1B/p27Kip and is involved in G1-S cell cycle transition in a CKS1-dependent manner.
- Goldenberg S.J. et al. (2004) Cell 119: 517
- Hristova V.A. et al. (2012) Mol. Cell 47: 331
- Tedesco D. et al. (2002) Genes & Dev. doi:10.1101/gad.1011202
- Wang W. et al. (2005) Biochemistry doi.org/10.1021/bi051071j
- Wu G. et al. (2003) Mol. Cell 11: 1445
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