Recombinant Human SKP1/SKP2 Complex Protein, CF

Catalog # Availability Size / Price Qty
E3-521-025
Product Details
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Recombinant Human SKP1/SKP2 Complex Protein, CF Summary

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Typical protein concentration for use in vitro will depend on experimental conditions.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human SKP1/SKP2 Complex protein
Predicted Molecular Mass
19 kDa (SKP1), 48 kDa (SKP2)

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-521

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 36 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SKP1/SKP2 Complex

SKP1 (S-phase kinase-associated protein 1) is an essential component of SCF (SKP1-CUL1-F-box protein) E3 Ubiquitin ligases that mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.  SKP1 is the adapter protein that links Cullin-1 to various F-box proteins (such as SKP2) that serve as the substrate recognition components of SCF ligases.  SKP2 targets include proteins involved in cell cycle progression and signal transduction.  SKP2 recognizes phosphorylated CDKN1B/p27Kip and is involved in G1-S cell cycle transition in a CKS1-dependent manner.

References
  1. Goldenberg S.J. et al. (2004) Cell  119: 517
  2. Hristova V.A. et al. (2012) Mol. Cell  47: 331
  3. Tedesco D. et al. (2002) Genes & Dev.  doi:10.1101/gad.1011202
  4. Wang W. et al. (2005) Biochemistry  doi.org/10.1021/bi051071j
  5. Wu G. et al. (2003) Mol. Cell  11: 1445
Long Name
S-phase Kinase Associated Protein 1/S-phase Kinase Associated Protein 2 Complex
Entrez Gene IDs
6500 (Human)
Alternate Names
SKP1/SKP2 Complex

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