Recombinant Human Slit1 Protein, CF

Catalog # Availability Size / Price Qty
6514-SL-050
Product Details
Citations (2)
FAQs
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Recombinant Human Slit1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Optimal neurite outgrowth was observed when neurons were plated on 96-well plates that had been pre-coated with 50 μL/well of Recombinant Human Slit1 at 2.5 ‑ 10 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Slit1 protein
Met1-Gln915, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Cys34
Predicted Molecular Mass
98.8 kDa
SDS-PAGE
105-125 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6514-SL

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Slit1

Slit1 is a member of the Slit family of large secreted axon guidance molecules that are ligands for Robo receptors (1, 2). Like other mammalian family members, the 1534 amino acid (aa), ~200 kDa human Slit1 contains a 33 aa signal sequence followed by 23 leucine-rich repeats (LRR, aa 34‑900) and 9 EGF-like sequences (aa 930‑1454) (2). Mammalian Slits also contain a laminin-G domain between EGF6 and EGF7 (aa 1166‑1339), and a C‑terminal cysteine-rich domain (cysteine knot; aa 1459‑1534) (2). Heparin sulfates are required for interaction of Robo with Slit LRR domains (2, 3). Within the LRR domains, human Slit1 shares 96% aa identity with mouse, rat, and bovine, 98% with equine and 91% with canine Slit1. Human Slits 1, 2 and 3 share 68-74% aa identity within the LRR domains. One potential isoform  of 1641 aa has 10 aa inserted after aa 338 within LRR9 and lacks aa 793‑816 within LRR20-21 (4). Two more potential isoforms of 1520 and 1461 aa diverge at the C‑terminus (aa 1453 and 1411, respectively); these isoforms lack the cysteine knot, which may mediate interaction with other proteins (5). Slit1 has been found mainly, but not exclusively, in the fetal and adult brain (2). Slit1 and Slit2 (or in some cases Slit3) are expressed in complementary locations during development of the optic and olfactory tracts and the forebrain, and appear to work together to mediate Robo guidance of retinal, olfactory, hippocampal and motor axons (1, 6‑11). Deletion of either Slit1 or Slit2 has less effect than deletion of both, which allows axons to wander from tracts and inappropriately cross or recross the midline (6, 7, 9‑11). Expression of Slit1 by new neurons influences astrocytes to form and maintain tunnels that guide neuronal migration (12). In the injured spinal cord, presence of Slit1 along with Slit3 and Netrin-1 may be responsible for failure of axons to regenerate in the adult CNS (13). Slit1 also promotes dendrite growth and branching of cortical neurons indicating it may exert important influence on the final morphology of cortical neurons (14).

References
  1. Yuan, W. et al. (1999) Dev. Biol. 212:290.
  2. Hohenester, E. (2008) Biochem. Soc. Trans. 36:251.
  3. Hussain, S-A. et al. (2006) J. Biol. Chem. 281:39693.
  4. Accession # EAW49957.
  5. Accession # EAW49956 and CAH70917.
  6. Thompson, H. et al. (2006) Dev. Biol. 296:476.
  7. Plump, A. S. et al. (2002) Neuron 33:219.
  8. Cho, J. H. et al. (2007) J. Neurosci. 27:9094.
  9. Nguyen-Ba-Charvet, K. T. et al. (2002) J. Neurosci. 22:5473.
  10. Bagri, A. et al. (2002) Neuron 33:233.
  11. DiMeglio, T. et al. (2008) J. Neurosci. 28:6285.
  12. Kaneko, N. et al. (2010) Neuron 67:213.
  13. Wehrle, R. et al. (2005) Eur. J. Neurosci. 22:2134.
  14. Whitford, K. L. et al. (2002) Neuron 33:47.
Long Name
SLIT Homolog 1
Entrez Gene IDs
6585 (Human); 20562 (Mouse)
Alternate Names
KIAA0813; MEGF4; MEGF4slit (Drosophila) homolog 1; MGC164811; Multiple EGF-like domains protein 4; Multiple epidermal growth factor-like domains protein 4; SLIL1; slit homolog 1 (Drosophila); slit homolog 1 protein; Slit1; Slit-1slit1; SLIT3

Citations for Recombinant Human Slit1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Effects of neuroactive agents on axonal growth and pathfinding of retinal ganglion cells generated from human stem cells
    Authors: T Yokoi, T Tanaka, E Matsuzaka, F Tamalu, SI Watanabe, S Nishina, N Azuma
    Sci Rep, 2017;7(1):16757.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. A PML/Slit Axis Controls Physiological Cell Migration and Cancer Invasion in the CNS
    Authors: V Amodeo, D A, J Betts, S Bartesaghi, Y Zhang, A Richard-Lo, M Ellis, R Roshani, M Vouri, S Galavotti, S Oberndorfe, AP Leite, A Mackay, A Lampada, E Wessel Str, N Li, D Dinsdale, D Grimwade, C Jones, P Nicotera, D Michod, S Brandner, P Salomoni
    Cell Rep, 2017;20(2):411-426.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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