Recombinant Human SLITRK3 Protein, CF

Catalog # Availability Size / Price Qty
8957-SK-050

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Recombinant Human SLITRK3 Protein, CF Summary

Product Specifications

Purity
>75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 200 ng on a nitrocellulose-coated microplate.
Source
Mouse myeloma cell line, NS0-derived human SLITRK3 protein
Thr29-Val651, with substitutions of Arg425Ser, Asn490Tyr, Phe510Leu and a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Thr29
Predicted Molecular Mass
71 kDa
SDS-PAGE
80-108 kDa, reducing conditions

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8957-SK

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8957-SK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: SLITRK3

SLITRK3 (Slit and Trk-like family member 3) is an approximately 120 kDa type I transmembrane member of the SLITRK family of proteins which contain a Slit-like extracellular region and (save for SLITRK1) a Trk-like cytoplasmic region (1). The extracellular domain (ECD) of mature human SLITRK3 contains 6 leucine rich repeats (LRR) followed by a C-terminal LRR domain, followed by 6 more LRR flanked by a pair of N- and C-terminal LRR domains (2, 3). Within the ECD, human SLITRK3 shares 98% amino acid sequence identity with mouse and rat SLITRK3. It is expressed in multiple regions of the brain, particularly the cerebral cortex and hippocampus (4). SLITRK3 can suppress neurite outgrowth and promote the formation of inhibitory presynaptic structures (3-5). It localizes post-synaptically and binds to PTP-delta and PTP-sigma on pre-synaptic inhibitory terminals (4, 5). SLITRK3 is upregulated during the development of gastrointestinal stromal tumors (6).

References
  1. Ko, J. (2012) Mol. Cells 34:335.
  2. Aruga, J. et al. (2003) Gene 315:87.
  3. Aruga, J. and K. Mikoshiba (2003) Mol. Cell Neurosci. 24:117.
  4. Yim, Y.S. et al. (2013) Proc. Natl. Acad. Sci. USA 110:4057.
  5. Takahashi, H. et al. (2012) Nat. Neurosci. 15:389.
  6. Wang, C.-J. et al. (2015) World J. Gastroenterol. 21:8398.
Long Name
SLIT and NTRK-like Family, Member 3
Entrez Gene IDs
22865 (Human); 386750 (Mouse); 310519 (Rat)
Alternate Names
MGC138681; SLIT and NTRK-like family, member 3; SLITRK3

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