Recombinant Human SLITRK4 Protein, CF

Catalog # Availability Size / Price Qty
8945-SK-050
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Recombinant Human SLITRK4 Protein, CF Summary

Product Specifications

Purity
>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 90 ng on a nitrocellulose-coated microplate.
Source
Mouse myeloma cell line, NS0-derived human SLITRK4 protein
Asp19-Pro616, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Asp19
Predicted Molecular Mass
68 kDa
SDS-PAGE
87-105 kDa, reducing conditions

Product Datasheets

You must select a language.

x

8945-SK

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8945-SK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: SLITRK4

SLITRK4 (Slit and Trk-like family member 4) is an approximately 90 kDa type I transmembrane member of the SLITRK family of proteins which contain a slit-like extracellular region and (save for SLITRK1) a Trk-like cytoplasmic region (1). The extracellular domain (ECD) of mature human SLITRK4 contains 6 leucine rich repeats (LRR) followed by a C-terminal LRR domain, followed by 6 more LRR flanked by a pair of N- and C-terminal LRR domains (2, 3). Within the ECD, human SLITRK4 shares 98% amino acid sequence identity with mouse and rat SLITRK4. It is expressed in the testis and multiple regions of the brain, particularly the cerebral cortex, cerebellum, and hippocampus (4). SLITRK4 can suppress neurite outgrowth and promote the formation of excitatory presynaptic structures (3-5).

References
  1. Ko, J. (2012) Mol. Cells 34:335.
  2. Aruga, J. et al. (2003) Gene 315:87.
  3. Aruga, J. and K. Mikoshiba (2003) Mol. Cell Neurosci. 24:117.
  4. Yim, Y.S. et al. (2013) Proc. Natl. Acad. Sci. USA 110:4057.
  5. Takahashi, H. et al. (2012) Nat. Neurosci. 15:389.
Long Name
SLIT and NTRK-like Family, Member 4
Entrez Gene IDs
139065 (Human); 245446 (Mouse)
Alternate Names
DKFZp547M2010; SLIT and NTRK-like family, member 4; SLIT and NTRK-like protein 4; slit and trk like gene 4; SLITRK4

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs
Loading...

Reviews for Recombinant Human SLITRK4 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human SLITRK4 Protein, CF and earn rewards!

Have you used Recombinant Human SLITRK4 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review