Recombinant Human TAFA2/FAM19A2 Protein, CF

Catalog # Availability Size / Price Qty
4179-TA-050
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Recombinant Human TAFA2/FAM19A2 Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. rhTAFA2, immobilized at 6-24 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.
Source
E. coli-derived human TAFA2/FAM19A2 protein
Ala31-His131
Accession #
N-terminal Sequence
Analysis
Ala31
Predicted Molecular Mass
11.3 kDa
SDS-PAGE
11 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4179-TA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 300 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: TAFA2/FAM19A2

TAFA2 (also FAM19A2) is a secreted, 11 kDa member of the FAM19/TAFA family of chemokine-like proteins (1). It is synthesized as a 131 amino acid (aa) precursor that contains a 30 aa signal sequence and a 101 aa mature chain (SwissProt #: Q8N3H0). Like other members of the FAM19/TAFA family, with the exception of TAFA5, mature TAFA1 contains 10 regularly spaced cysteine residues that follow the pattern CX7CCX13CXCX14CX11CX4CX5CX10C, where C represents a conserved cysteine residue and X represents any noncysteine amino acid (1). Human TAFA2 is 97% aa identical to mouse TAFA2 (1). TAFA2 expression can be detected in the central nervous system (CNS), colon, heart, lung, spleen, kidney, and thymus, but its expression in the CNS is 50- to 1000-fold higher than in other tissues (1). Within the CNS, TAFA2 expression is highest in the occipital and frontal cortex (3- to 10-fold more abundantly expressed than in other cortical regions) and medulla (1). The biological functions of TAFA family members remain to be determined, but there are a few tentative hypotheses. First, TAFAs may modulate immune responses in the CNS by functioning as brain-specific chemokines, and may act with other chemokines to optimize the recruitment and activity of immune cells in the CNS (1). Second, TAFAs may represent a novel class of neurokines that act as regulators of immune nervous cells (1 - 2). Finally, TAFAs may control axonal sprouting following brain injury (1).

References
  1. Tang, Y.T. et al. (2004) Genomics 83:727.
  2. Benveniste, E. (1998) Cytokine Growth Factor Rev. 9:259.
Long Name
Family with Sequence Similarity 19, Member A2
Entrez Gene IDs
338811 (Human); 268354 (Mouse)
Alternate Names
Chemokine-like protein TAFA-2; DKFZp761E1217; DKFZp781P0552; FAM19A2; family with sequence similarity 19 (chemokine (C-C motif)-like), member A2; MGC42403; TAFA2; TAFA-2

Citations for Recombinant Human TAFA2/FAM19A2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. TAFA2 induces skeletal (stromal) stem cell migration through activation of Rac1-p38 signaling
    Authors: A Jafari, A Isa, L Chen, N Ditzel, W Zaher, L Harkness, HE Johnsen, BM Abdallah, C Clausen, M Kassem
    Stem Cells, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Human TAFA2/FAM19A2 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human TAFA2/FAM19A2 Protein, CF and earn rewards!

Have you used Recombinant Human TAFA2/FAM19A2 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review