Recombinant Human TfR2 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
Recombinant Human TfR2 Fc Chimera Protein Binding Activity
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Product Details

Recombinant Human TfR2 Fc Chimera Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Bioassay data are not available.
Chinese Hamster Ovary cell line, CHO-derived human TfR2 protein
MDHuman IgG1
IEGRHuman TfR2
Accession # NP_003218.2
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
103 kDa
105-117 kDa, under reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human TfR2 Fc Chimera Protein Binding Activity View Larger

In a Goat Anti-Human IgG Fc Antibody (G-102-C) coated plate, Recombinant Human TfR2 Fc Chimera (10486-TF) binds to Biotinylated Human Transferrin with an ED50 of 0.1-0.6 μg/mL.

SDS-PAGE Recombinant Human TfR2 Fc Chimera Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Human TfR2 Fc Chimera Protein (Catalog # 10486-TF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-117 kDa and 180-210 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: TfR2

Transferrin Receptor 2 (TfR2 or TfR2 alpha) is an iron-binding type 2 transmembrane glycoprotein homologous to the Transferrin Receptor 1 (TfR or TfR1) (1). It forms a 215 kDa disulfide-linked homodimer and is predominantly expressed in the liver and modulates hepcidin production in response to iron (1, 2). Human TfR2 cDNA encodes 801 amino acids (aa) including an 83 aa N-terminal intracellular region, a 21 aa transmembrane domain, and a 697 aa extracellular domain (ECD). Human TfR2 ECD shares 86% and 45% aa identity with mouse TfR2 and human TfR1, respectively. An alternative splicing isoform (TfR2 beta) lacks the intracellular region, transmembrane domain and part of the extracellular regions and is expressed ubiquitously (1, 3). The Iron loaded Transferrin (holo-Transferrin) circulating in blood binds to cell-surface receptor TFR2 (4). Mutations in TFR2 can cause a form of hereditary hemochromatosis (HH, type 111), a disorder marked by chronic iron overload (5). 

  1. Kawabata, H. et al. (1999) J. Biol. Chem. 274:20826.
  2. Trinder, D. and E. Baker (2003) Int. J. Biochem. Cell. Biol. 35:292.
  3. Deaglio, S. et al. (2002) Blood 100:3782.
  4. Aisen, P. et al. (1978) J. Biol Chem. 253:1930.
  5. Camaschella, C. et al. (2000) Nat Genet. 25:14.
Long Name
Transferrin Receptor 2
Entrez Gene IDs
7036 (Human); 50765 (Mouse)
Alternate Names
HFE3; HFE3transferrin receptor protein 2; MGC126368; TfR2; TFRC2; transferrin receptor 2


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