Recombinant Human TIGIT (T103) His-tag Protein, CF Summary
Met22-Ala136 (T103), with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and Trehalose.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human CD155/PVR Fc Chimera (Catalog # 9174-CD) is coated at 2.5 µg/mL, Recombinant Human TIGIT (T103) His-Tag (Catalog # 9525-TG) binds with an ED50 of 0.3-1.5 µg/mL.
TIGIT (T cell Immunoreceptor with Ig and ITIM domains), also called Vstm3 (V-set and transmembrane domain-containing 3), Vsig9 (V-set and Ig domain-containing 9) and WUCAM (Washington University cell adhesion molecule) is a 30-34 kDa type I transmembrane protein that is a member of the CD28 family within the Ig superfamily of proteins (1-4). Human TIGIT cDNA encodes 244 amino acids (aa) including a 21 aa signal sequence, a 120 aa extracellular region with a V-type Ig-like domain and two potential N-glycosylation site, a 21 aa transmembrane sequence, and an 82 aa cytoplasmic domain with an ITIM motif (5). Within the ECD, human TIGIT shares only 65% and 67% aa sequence identity with mouse and rat TIGIT. TIGIT is expressed on NK cells and subsets of activated, memory, and regulatory T cells, and particularly on follicular helper T cells within secondary lymphoid organs (1, 2, 6-8). It binds to CD155/PVR/Necl-5 and Nectin-2/CD112/PVRL2 that appear on dendritic cells (DC) and endothelium (1-3, 7). Binding of TIGIT by DC induces IL-10 release and inhibits IL-12 production (2). Ligation of TIGIT on T cells down‑regulates TCR-mediated activation and subsequent proliferation, while in NK cell, TIGIT ligation blocks NK cell cytotoxicity (6-8). Through CD155 and Nectin-2, which also interact with DNAM-1/CD226 and CD96/Tactile, TIGIT is part of an interacting network of Ig superfamily members that may augment or oppose each other (3, 4, 6, 7). In particular, TIGIT binding to CD155 can antagonize the effects of DNAM-1 (6, 7). Soluble TIGIT is able to compete with DNAM-1 for CD155 binding and attenuates T cell responses, while mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges (2, 3, 8).
- Boles, K.S. et al. (2009) Eur. J. Immunol. 39:695.
- Yu, X. et al. (2009) Nat. Immunol. 10:48.
- Levin, S.D. et al. (2011) Eur. J. Immunol. 41:902.
- Xu, Z. et al. (2010) Cell. Mol. Immunol. 7:11.
- SwissProt Accession # Q495A1.
- Seth, S. et al. (2009) Eur. J. Immunol. 39:3160.
- Stanietsky, N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:17858.
- Joller, N. et al. (2011) J. Immunol. 83:1338.
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Human TIGIT (T103) His-tag Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human TIGIT (T103) His-tag Protein, CF and earn rewards!
Have you used Recombinant Human TIGIT (T103) His-tag Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image