Recombinant Human TINAGL1 Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Cultrex Mouse Laminin I (Catalog # 3400-010-01) is coated at 5 µg/mL, Recombinant Human TINAGL1 binds with an ED50 = 1-6 ng/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived
    Human TINAGL1
    (Ala22-Met464)
    Accession # Q9GZM7
    HPGGGSGGGSGGGSHHHHHH
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala22
  • Predicted Molecular Mass
    52 kDa
  • SDS-PAGE
    47-61 kDa, reducing conditions
9304-TU
 
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Brij-35.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Data Images
When Cultrex MouseLaminin I (Catalog #
3400-010-01) is coated at 5 µg/mL, Recombinant HumanTINAGL1 (Catalog # 9304-TU) binds with an ED50 = 1-6 ng/mL.
Background: TINAGL1
TINAGL1 (Tubulointerstitial nephritis antigen-like 1; also TIN-ag-RP, AZ-1 and Lipocalin 7) is a 54-57 kDa glycoprotein member of the peptidase C1 family of molecules. Mature human TINAGL1 contains an SMB (somatomedin B) domain, one vWFC domain, and a nonenzymatic peptidase C1A region (1). Mature human TINAGL1 shares 90% amino acid (aa) identity with mouse TINAGL1. Alternative splicing generates additional isoforms that either lack a portion of the vWFC domain or consist of a portion of the peptidase domain with a 38 aa insertion and flanked by substitutions (2). TINAGL1 is secreted by renal tubule epithelium, zona glomerulosa cells, vascular smooth muscle, and striated muscle cells (1, 3, 4). It is also expressed in mouse decidual endometrium and blastocysts (5-7). During pregnancy it binds to Laminin-1 and Integrins alpha 5 and beta 1 and plays a role in supporting fertility (6-8). In the vasculature, TINAGL1 localizes to basal laminae along adrenocortical sinusoidal capillaries where it binds to fibronectin and collagen and supports adrenocortical cell adhesion (9).
  • References:
    1. Wex, T. et al. (2001) Biochemistry 40:1350.
    2. Zhou, B. et al. (2000) J. Am. Soc. Nephrol. 11:658.
    3. Bromme, N.C. et al. (2000) Biochem. Biophys. Res. Commun. 271:474.
    4. Mukai, K. et al. (2003) J. Biol. Chem. 278:17084.
    5. Sakurai, M. et al. (2014) Zygote 22:259.
    6. Tajiri, Y. et al. (2010) Biol. Reprod. 82:263.
    7. Igarashi, T. et al. (2009) Biol. Reprod. 81:948.
    8. Takahashi, A. et al. (2016) J. Reprod. Dev. 62:43.
    9. Li, D. et al. (2007) FEBS J. 274:2506.
  • Long Name:
    Tubulointerstitial Nephritis Antigen-like 1
  • Entrez Gene IDs:
    64129 (Human); 94242 (Mouse); 94174 (Rat)
  • Alternate Names:
    androgen-regulated gene 1; ARG1; GIS5; Glucocorticoid-inducible protein 5; LCN7; LIECG3; lipocalin 7; OLRG2; OLRG-2; Oxidized LDL-responsive gene 2 protein; oxidized-LDL responsive gene 2; P3ECSL; TIN Ag-related protein; TINAGL; TINAGL1; TINAG-like 1; TINAGRP; TIN-ag-RP; tubulointerstitial nephritis antigen-like 1; tubulointerstitial nephritis antigen-like; Tubulointerstitial nephritis antigen-related protein
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Biotinylated Antibody

WB BAM050 3  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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