Recombinant Human TRAILR4/TNFRSF10D Fc Chimera Protein, CF

R&D Systems | Catalog # 633-TR

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human TRAILR4/TNFRSF10D Fc Chimera Protein (633-TR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q9UBN6

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all TRAILR4/TNFRSF10D Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human TRAILR4/TNFRSF10D protein
Human TRAIL R4
(Ala56 - His211)
Accession # Q9UBN6		 IEGRID Human IgG1
(Pro100 - Lys330)		 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala56

Predicted Molecular Mass

44.2 kDa (monomer)

SDS-PAGE

70-80 kDa, reducing conditions

Activity

Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL.
The ED50 for this effect is 6.00-60.0 ng/mL in the presence of 20 ng/mL of rhTRAIL.

Formulation, Preparation, and Storage

633-TR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRAILR4/TNFRSF10D

Human TRAIL R4, also called decoy receptor 2 (DcR2) and TRUNND (TRAIL receptor with a truncated death domain), is a type 1, TNF R family membrane protein, which is a receptor for TRAIL (APO2 ligand). In the new TNF superfamily nomenclature, TRAIL R4 is referred to as TNFRSF10D. TRAIL R4 is unique among the TRAIL receptors in that its cytoplasmic domain contains a truncated consensus death domain motif. Binding of TRAIL R4 does not result in an apoptotic signal. Overexpression of TRAIL R4 can protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-mediated apoptosis. The human soluble TRAIL R4/Fc chimera neutralizes the ability of TRAIL to induce apoptosis.

References

  1. Griffith, T.S. et al. (1998) Curr. Opin. Immunol. 10:559.
  2. Pan, G. et al. (1998) FEBS lett 424:41.
  3. Marsters, S.A. et al. (1997) Cur. Biol. 7:1003.
  4. Degli-Esposti, M.A. et al. (1997) Immunity 7:813.

Long Name

TRAIL Receptor 4

Alternate Names

CD264, DcR2, TNFRSF10D, TRAIL R4

Entrez Gene IDs

8793 (Human)

Gene Symbol

TNFRSF10D

UniProt

Q9UBN6

Additional TRAILR4/TNFRSF10D Products

Product Documents for Recombinant Human TRAILR4/TNFRSF10D Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human TRAILR4/TNFRSF10D Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human TRAILR4/TNFRSF10D Fc Chimera Protein, CF

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