Recombinant Human TREM-2 His-tag Protein, CF

  
  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to bind fluorescein-conjugated E. coli Bioparticles. The ED50 for this effect is 0.15-0.9 μg/mL using a His Tag Antibody coated plate.
  • Source
    Mouse myeloma cell line, NS0-derived His19-Ser174, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    His19
  • Predicted Molecular Mass
    18 kDa
  • SDS-PAGE
    25-39 kDa, reducing conditions
9256-T2
 
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human TREM-2 (Catalog #
9256-T2) bindsfluorescein-conjugated E.coli Bioparticles. The ED50 for this effect is0.15-0.9 μg/mL using an anti-His Tag Antibody (Catalog # MAB050) coatedplate.
Background: TREM-2
TREM-2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa type I transmembrane member of the TREM family and Ig superfamily (1). Mature human TREM-2  consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail (2). Within the ECD, human TREM-2 shares 73% and 74% aa sequence identity with mouse and rat TREM-2, respectively. Soluble forms of the TREM-2 ECD are generated by alternative splicing or proteolytic cleavage, and the cytoplasmic domain can be liberated by gamma-Secretase mediated intramembrane cleavage (3). A positively charged lysine within the transmembrane segment allows association with the signal adapter protein, DAP12 and inhibition of macrophage activation (4, 5). TREM-2 is expressed on macrophages, immature myeloid dendritic cells, osteoclasts, microglia, and adipocytes (5-9). It promotes the differentiation and function of osteoclasts, the production of inflammatory cytokines by adipocytes, insulin resistance, and the phagocytic clearance of bacteria (9-11). In the CNS, TREM-2 binds to ApoE, ApoA1, and ApoB and mediates the clearance of apoptotic neurons, amyloid plaques, and cell debris following demyelination (6-8, 12). TREM-2 also interacts with and modifies signaling through Plexin A1 on dendritic cells and osteoclasts (13). Mutations in TREM-2 or DAP12 are associated with the development of Alzheimer's disease and Nasu-Hakola disease (NHD/PLOSL) which is characterized by presenile dementia and bone cysts (14, 15). Soluble TREM-2 is elevated in cerebrospinal fluid of patients with active multiple sclerosis (MS), and TREM-2 blockade exacerbates disease symptoms in the experimental EAE model of MS (16, 17).
  • References:
    1. Painter, M.M. et al. (2015) Mol. Neurodegener. 10:43.
    2. Bouchon, A. et al. (2000) J. Immunol. 164:4991.
    3. Wunderlich, P. et al. (2013) J. Biol. Chem. 288:33027.
    4. Hamerman, J. A. et al. (2006) J. Immunol. 177:2051.
    5. Turnbull, I.R. et al. (2006) J. Immunol. 177:3520.
    6. Takahashi, K. et al. (2005) J. Exp. Med. 201:647.
    7. Atagi, Y. et al. (2015) J. Biol. Chem. 290:26043.
    8. Wang, Y. et al. (2016) J. Exp. Med. 213:667.
    9. Cella, M. et al. (2003) J. Exp. Med. 198:645.
    10. Park, M. et al. (2015) Diabetes 64:117.
    11. N'Diaye, E-N. et al. (2009) J. Cell Biol. 184:215.
    12. Poliani, P.L. et al. (2015) J. Clin. Invest. 125:2161.
    13. Takegahara, N. et al. (2006) Nat. Cell Biol. 8:615.
    14. Colonna, M. and Y. Wang (2016) Nat. Rev. Neurosci. 17:201.
    15. Paloneva, J. et al. (2002) Am. J. Hum. Genet. 71:656.
    16. Piccio, L. et al. (2008) Brain 131:3081.
    17. Piccio, L. et al. (2007) Eur. J. Immunol. 37:1290.
  • Long Name:
    Triggering Receptor Expressed on Myeloid Cells 2
  • Entrez Gene IDs:
    54209 (Human)
  • Alternate Names:
    TREM2; TREM-2; Trem2a; Trem2b; Trem2c; TREM-2triggering receptor expressed on myeloid cells 2a; Triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2
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