Recombinant Human TROP-2 Fc Chimera Protein, CF

R&D Systems | Catalog # 650-T2

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human TROP-2 Fc Chimera Protein (650-T2)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P09758.3

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all TROP-2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human TROP-2 protein
Human TROP-2
(Gln-His27-Thr274)
Accession # P09758.3		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln (blocked)-His27, Gln31 (blocked) & Thr88

Predicted Molecular Mass

49.2 kDa (monomer)

SDS-PAGE

61-76 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells or LS180 human colorectal adenocarcinoma cells.
When 1 x 104 to 5 x 104 cells/well are added to Recombinant Human TROP‑2 Fc Chimera coated plates (10 µg/mL with 100 µL/well), >45% cells will adhere after 1 hour incubation.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation, and Storage

650-T2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TROP-2

Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

References

  1. Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.
  2. Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.
  3. Fornaro, M. et al. (1995) Int. J. Cancer 62:610.
  4. Ripani, E. et al. (1998) Int. J. Cancer 76:671.
  5. El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.

Long Name

Tumor-associated Calcium Signal Transducer 2

Alternate Names

GA733-1, gp50, T16, TACSTD2, TROP2

Entrez Gene IDs

4070 (Human); 56753 (Mouse)

Gene Symbol

TACSTD2

UniProt

P09758.3

Additional TROP-2 Products

Product Documents for Recombinant Human TROP-2 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human TROP-2 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human TROP-2 Fc Chimera Protein, CF

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