Recombinant Human TSPAN8-LEL Fc Chimera Protein, CF

R&D Systems | Catalog # 11550-TS

Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems CHO-derived Recombinant Human TSPAN8-LEL Fc Chimera Protein (11550-TS)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all TSPAN8 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human TSPAN8 protein
MD Human IgG1
(Pro100-Lys330)
 IEGRM Human TSPAN8-LEL
(Lys110-Asn205)
Accession # P19075.1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

38 kDa

SDS-PAGE

40-50 kDa, under reducing conditions

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant Human TSPAN8-LEL Fc Chimera Protein, CF

Recombinant Human TSPAN8-LEL Fc Chimera Protein SEC-MALS.

Recombinant Human TSPAN8-LEL Fc Chimera (Catalog # 11550-TS) has a molecular weight (MW) of 82.6 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (e.g. Glycosylation).

Recombinant Human TSPAN8-LEL Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human TSPAN8-LEL Fc Chimera Protein (Catalog # 11550-TS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-50 kDa and 80-100 kDa, respectively.

Formulation, Preparation, and Storage

11550-TS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TSPAN8

Tetraspanin 8, or TSPAN8, belongs to a superfamily of proteins that is characterized by four transmembrane domains, three intracellular domains and two extracellular loops: a small extracellular loop (SEL) and a large extracellular loop (LEL).   The extracellular loops form molecular webs that bring together cell surface proteins, facilitating the formation of stable and functional signalling complexes. Tetraspanins form microdomains on the plasma membrane that mediate diverse biological processes including adhesion, cell fusion, immune response, and tumor development (1-4).  Human TSPAN8 consists of 237 amino acids, with the LEL region spanning residues 110-205.  Within the LEL, human TSPAN8 shares 57% aa sequence identity with mouse and rat TSPAN8-LEL.

References

  1. Charrin, S. et al. (2014) J. Cell Sci. 127:3641.
  2. Yang, J. et al. (2024) Cell. 13:193.
  3. Hemler, M.E. (2005) Nat. Rev. Mol. Cell Biol.6:801.
  4. Kim, T-K. et al. (2015) Biochem. Biophys. Res. Commun. 468:774.

Long Name

Tetraspanin 8/Transmembrane 4 Superfamily, Member 3

Alternate Names

CO-029, TM4SF3

Entrez Gene IDs

7103 (Human); 216350 (Mouse); 171048 (Rat)

Gene Symbol

TSPAN8

Additional TSPAN8 Products

Product Documents for Recombinant Human TSPAN8-LEL Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human TSPAN8-LEL Fc Chimera Protein, CF

For research use only

Related Research Areas

Integrins and Associated Molecules

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