Recombinant Human TWEAK/TNFSF12 Protein, CF

(7 citations)   
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 2-8 ng/mL.
  • Source
    E. coli-derived Arg93-His249, with an N-terminal Met and 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    18.3 kDa
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1090-TW/CF
 
1090-TW
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human TWEAK/TNFSF12 (Catalog # 1090-TW/CF) stimulates cell proliferation in HUVEC human umbilical vein endothelial cells. The ED50 is 2‑8 ng/mL.
1 μg/lane of Recombinant Human TWEAK/TNFSF12 was resolved with
SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.
Background: TWEAK/TNFSF12

TWEAK is a type II transmembrane protein belonging to the TNF superfamily (1). It contains a short cytoplasmic domain (aa 1‑18), the transmembrane domain
(aa 19-42) and an extracellular domain (aa 43-249). The extracellular domains of human and murine TWEAK share 89% aa sequence identity. A soluble form of TWEAK is generated from the membrane-associated molecules by proteolytic cleavage after Arg 93 suggesting that TWEAK may have long-range effects. TWEAK is expressed widely in many tissues and cells. At least two receptors that bind TWEAK have been identified (2-4). Death Receptor 3 (DR3), also known as TNFRSF12, Apo-3, LARD, WSL-1 or TRAMP, is a TNF receptor superfamily member that is expressed predominantly in tissues with high lymphocyte content (2). It has been suggested that induction of cell death by TWEAK-DR3 interaction involves the activation of NF-kappa B. In cells that lack DR3, alternate pathways of TWEAK-induced cell death mediated by receptors distinct from DR3 have been suggested (5, 6). TWEAK receptor (TWEAKR, alternatively known as FN14), is a novel TNF receptor superfamily member that also binds TWEAK (3, 4). It is a mitogen-inducible gene that is expressed in fibroblasts, hepetocellular carcinomas and endothelial cells. TWEAK-TWEAKR interaction has been shown to play a role in endothelial cell growth and migration. This effect of TWEAK is not mediated by an up-regulation of VEGF (7).

  • References:
    1. Chicheportiche, Y. et al. (1997) J. Biol Chem. 272:32401.
    2. Marsters, S. et al. (1998) Current Biol. 8:525.
    3. Wiley, S. R. et al. (2001) Immunity, 15:837.
    4. Feng, S.-L.Y. et al. (2000) Am J. Path. 156:1253.
    5. Nakayama, M. et al. (2002) J. Immunol. 168:734.
    6. Schneider, P. et al. (1999) Eur. J. Immunol., 29:1785.
    7. Lynch, C. et al. (1998) J. Biol. Chem. 274:8455.
  • Long Name:
    TNF-like and Weak Inducer of Apoptosis
  • Entrez Gene IDs:
    8742 (Human); 21944 (Mouse); 360548 (Rat)
  • Alternate Names:
    APO3 ligand; APO3LMGC20669; DR3LGTWEAKAPO3/DR3 ligand; MGC129581; TNF-related weak inducer of apoptosis; TNFSF12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand superfamily member 12; TWEAK
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Biliary tract instillation of a SMAC mimetic induces TRAIL-dependent acute sclerosing cholangitis-like injury in mice
    Authors: ME Guicciardi, A Krishnan, SF Bronk, P Hirsova, TS Griffith, GJ Gores
    Cell Death Dis, 2017;8(1):e2535.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Down-regulation of Forkhead Box Protein A1 (FOXA1) Leads to Cancer-stem Cell-like Properties in Tamoxifen-resistant Breast Cancer Cells through Induction of Interleukin-6
    Authors: N Yamaguchi, Y Nakayama, N Yamaguchi
    J. Biol. Chem, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. TWEAK mediates inflammation in experimental atopic dermatitis and psoriasis
    Authors: D Sidler, P Wu, R Herro, M Claus, D Wolf, Y Kawakami, T Kawakami, L Burkly, M Croft
    Nat Commun, 2017;8(0):15395.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Tumor Necrosis Factor-Like Weak Inducer of Apoptosis Promotes Hepatic Stellate Cells Migration via Canonical NF-?B/MMP9 Pathway
    PLoS ONE, 2016;11(12):e0167658.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. Regulation of fibroblast growth factor-inducible 14 (Fn14) expression levels via ligand-independent lysosomal degradation.
    Authors: Gurunathan S, Winkles J, Ghosh S, Hayden M
    J Biol Chem, 2014;289(19):12976-88.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. The TWEAK receptor Fn14 is a therapeutic target in melanoma: immunotoxins targeting Fn14 receptor for malignant melanoma treatment.
    Authors: Zhou H, Ekmekcioglu S, Marks J, Mohamedali K, Asrani K, Phillips K, Brown S, Cheng E, Weiss M, Hittelman W, Tran N, Yagita H, Winkles J, Rosenblum M
    J Invest Dermatol, 2013;133(4):1052-62.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Lipocalin 2, the TNF-like receptor TWEAKR and its ligand TWEAK act downstream of NFAT1 to regulate breast cancer cell invasion.
    Authors: Gaudineau B, Fougere M, Guaddachi F, Lemoine F, de la Grange P, Jauliac S
    J Cell Sci, 2012;125(0):4475-86.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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