Recombinant Human TWEAK/TNFSF12 Protein

R&D Systems | Catalog # 1090-TW

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human TWEAK/TNFSF12 Protein (1090-TW)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

Q4ACW9

Applications

Bioactivity
View all TWEAK/TNFSF12 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human TWEAK/TNFSF12 protein
Arg93-His249, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

18.3 kDa

Activity

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells.
The ED50 for this effect is 0.200-4.00 ng/mL.

Reviewed Applications

Read 3 reviews rated 4.3 using 1090-TW in the following applications:

Scientific Data Images for Recombinant Human TWEAK/TNFSF12 Protein

Recombinant Human TWEAK/TNFSF12 Protein Bioactivity

Recombinant Human TWEAK/TNFSF12 Protein Bioactivity

Recombinant Human TWEAK/TNFSF12 (Catalog # 1090-TW) stimulates cell proliferation in HUVEC human umbilical vein endothelial cells. The ED50 is 2-8 ng/mL.
Recombinant Human TWEAK/TNFSF12 Protein SDS-PAGE

Recombinant Human TWEAK/TNFSF12 Protein SDS-PAGE

1 μg/lane of Recombinant Human TWEAK/TNFSF12 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1090-TW
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1090-TW/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TWEAK/TNFSF12

TWEAK is a type II transmembrane protein belonging to the TNF superfamily (1). It contains a short cytoplasmic domain (aa 1‑18), the transmembrane domain
(aa 19-42) and an extracellular domain (aa 43-249). The extracellular domains of human and murine TWEAK share 89% aa sequence identity. A soluble form of TWEAK is generated from the membrane-associated molecules by proteolytic cleavage after Arg 93 suggesting that TWEAK may have long-range effects. TWEAK is expressed widely in many tissues and cells. At least two receptors that bind TWEAK have been identified (2-4). Death Receptor 3 (DR3), also known as TNFRSF12, Apo-3, LARD, WSL-1 or TRAMP, is a TNF receptor superfamily member that is expressed predominantly in tissues with high lymphocyte content (2). It has been suggested that induction of cell death by TWEAK-DR3 interaction involves the activation of NF-kappa B. In cells that lack DR3, alternate pathways of TWEAK-induced cell death mediated by receptors distinct from DR3 have been suggested (5, 6). TWEAK receptor (TWEAKR, alternatively known as FN14), is a novel TNF receptor superfamily member that also binds TWEAK (3, 4). It is a mitogen-inducible gene that is expressed in fibroblasts, hepetocellular carcinomas and endothelial cells. TWEAK-TWEAKR interaction has been shown to play a role in endothelial cell growth and migration. This effect of TWEAK is not mediated by an up-regulation of VEGF (7).

References

  1. Chicheportiche, Y. et al. (1997) J. Biol Chem. 272:32401.
  2. Marsters, S. et al. (1998) Current Biol. 8:525.
  3. Wiley, S. R. et al. (2001) Immunity, 15:837.
  4. Feng, S.-L.Y. et al. (2000) Am J. Path. 156:1253.
  5. Nakayama, M. et al. (2002) J. Immunol. 168:734.
  6. Schneider, P. et al. (1999) Eur. J. Immunol., 29:1785.
  7. Lynch, C. et al. (1998) J. Biol. Chem. 274:8455.

Long Name

TNF-like and Weak Inducer of Apoptosis

Alternate Names

TNFSF12

Entrez Gene IDs

8742 (Human); 21944 (Mouse); 360548 (Rat)

Gene Symbol

TNFSF12

UniProt

Q4ACW9

Additional TWEAK/TNFSF12 Products

Product Documents for Recombinant Human TWEAK/TNFSF12 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 1090-TW (with carrier)
Download SDS 1090-TW/CF (carrier free)

Product Specific Notices for Recombinant Human TWEAK/TNFSF12 Protein

For research use only

Citations for Recombinant Human TWEAK/TNFSF12 Protein

Customer Reviews for Recombinant Human TWEAK/TNFSF12 Protein (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
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1 Stars
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  • Recombinant Human TWEAK/TNFSF12 Protein
    Name: Abby Tyler
    Application: Immunoassay Standard
    Verified Customer | Posted 11/08/2019
    This was used as the ELISA calibrator with MAB1090 and AF1090 as the antibodies. It had good parallelism with human serum and plasma samples.
    Recombinant Human TWEAK/TNFSF12 Protein 1090-TW
  • Recombinant Human TWEAK/TNFSF12 Protein
    Name: Anonymous
    Application: TaqMan qPCR
    Verified Customer | Posted 05/31/2018
    Recombinant Human TWEAK/TNFSF12 Protein 1090-TW
  • Recombinant Human TWEAK/TNFSF12 Protein
    Name: Anonymous
    Application: MS characterization
    Verified Customer | Posted 02/07/2018
    Recombinant Human TWEAK/TNFSF12 Protein 1090-TW

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