Recombinant Human UbcH8/UBE2L6 Protein, CF

R&D Systems | Catalog # E2-644

R&D Systems
Discontinued Product
E2-644 has been discontinued. View all UbcH8 products.

Key Product Details

Source

E. coli

Accession Number

O14933

Applications

Enzyme Activity
View all UbcH8 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human UbcH8 protein

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

18 kDa

Activity

Recombinant Human UbcH8/UBE2L6 is a member of the Ub AND ISG15-conjugating (E2) enzyme family that receives Ub AND ISG15 from a Ub AND ISG15-activating (E1) enzyme and subsequently interacts with a Ub AND ISG15 ligase (E3) to conjugate Ub AND ISG15 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH8/UBE2L6 concentration of 0.1-1 μM.

Formulation, Preparation, and Storage

E2-644
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: UbcH8

Ubiquitin-conjugating Enzyme H8 (UbcH8), also known as Ubiquitin-conjugating Enzyme E2L 6 (UBE2L6), has a predicted molecular weight of 18 kDa. Human UbcH8 shares 76% and 74% amino acid sequence identity with mouse and rat UBE2L6, respectively. UbcH8 is capable of transferring either Ubiquitin or the Ubiquitin-like protein ISG15 Ubiquitin-like Modifier (ISG15) to target proteins (1,2). ISG15 conjugation is mediated by UbcH8 following IFN-alpha or IFN-beta stimulation (3,4). UbcH8 interacts with a variety of Ubiquitin ligases (E3s) to transfer Ubiquitin to target proteins such as the cell cycle regulator, p21/CIP1/CDKN1A (5). UbcH8 also regulates actin cytoskeleton dynamics, and dysregulated UbcH8 promotes migration and invasion of human breast cancer cells (6). In addition, UbcH8 interacts with the E3, Parkin, and is thought to play a role in the pathogenesis of Parkinson’s disease (1,7).

References

  1. Kumar, S. et al. (1997) J. Biol. Chem. 272:13548.
  2. Durfee, L.A. & J.M. Huibregste (2012) Methods Mol. Biol. 832:141.
  3. Zhao, C. et al. (2004) Proc. Natl. Acad. Sci. USA 101:7578.
  4. Kim, K. et al. (2004) Mol. Cell. Biol. 24:9592.
  5. Shibata, E. et al. (2011) Mol. Cell. Biol. 31:3136.
  6. Desai, S.D. et al. (2012) Exp. Biol. Med. 237:38.
  7. Zhang, Y. et al. (2000) Proc. Natl. Acad. Sci. USA 97:13354.

Alternate Names

RIG-B, UBE2L6

Entrez Gene IDs

9246 (Human); 56791 (Mouse); 295704 (Rat)

Gene Symbol

UBE2L6

UniProt

O14933 (Human)

Additional UbcH8 Products

Product Documents for Recombinant Human UbcH8/UBE2L6 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human UbcH8/UBE2L6 Protein, CF

For research use only

Customer Reviews for Recombinant Human UbcH8/UBE2L6 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human UbcH8/UBE2L6 Protein, CF and earn rewards!

Have you used Recombinant Human UbcH8/UBE2L6 Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes