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Recombinant Human Ubiquitin Aldehyde Protein, CF

Catalog #: U-201
17 Citations Datasheet / COA / SDS
 

Discontinued Product

U-201 has been discontinued.
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Citations (17)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Ubiquitin Aldehyde Protein, CF Summary

Product Specifications

Purity
>96%, by HPLC.
Activity
Add Recombinant Human Ubiquitin Aldehyde to in vitro assays to inhibit deubiquitinating enzymes. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin Aldehyde concentration of 2-5 μM.
Source
E. coli-derived human Ubiquitin protein
Contains a C-terminal Aldehyde
Accession #
P0CG47
Predicted Molecular Mass
8.6 kDa

Product Datasheets

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U-201

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

U-201

Formulation X mg/ml (X μM) as a solution in 50 mM HCl
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ubiquitin

Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1) (2,3). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting (4-7).

Ubiquitin Aldehyde is a potent and specific inhibitor of Ubiquitin C-terminal hydrolases (UCHs), Ubiquitin-specific proteases (USPs) and deubiquinating enzymes (DUBs). This protein blocks the hydrolysis of poly-Ubiquitin chains on substrate proteins in vitro and thus enhances poly-Ubiquitin chain accumulation.  Some DUBs, such as those of the JAMM class, are not inhibited by Ubiquitin Aldehyde.

References
  1. Sharp, P.M. & W.-H. Li. (1987) Trends Ecol. Evol. 2:328.
  2. Ciechanover, A. et al. (1980 ) Proc. Natl. Acad. Sci. USA 77:1365.
  3. Hershko, A. et al. (1980) Proc. Natl. Acad. Sci. USA 77:1783.
  4. Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
  5. Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
  6. Wei, W. et al. (2004) Nature 428:194.
  7. Wertz, I.E. et al. (2004) Nature 430:694.
Entrez Gene IDs
7314 (Human); 298693 (Rat)
Alternate Names
RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin

Citations for Recombinant Human Ubiquitin Aldehyde Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Regulated interaction of ID2 with the anaphase-promoting complex links progression through mitosis with reactivation of cell-type-specific transcription
    Authors: SB Lee, L Garofano, A Ko, F D'Angelo, B Frangaj, D Sommer, Q Gan, K Kim, T Cardozo, A Iavarone, A Lasorella
    Nature Communications, 2022-04-19;13(1):2089.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  2. Mammalian Ddi2 is a shuttling factor containing a retroviral protease domain that influences binding of ubiquitylated proteins and proteasomal degradation
    Authors: GA Collins, Z Sha, CL Kuo, B Erbil, AL Goldberg
    The Journal of Biological Chemistry, 2022-03-28;0(0):101875.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Neutralization
  3. The E3 ubiquitin ligase component, Cereblon, is an evolutionarily conserved regulator of Wnt signaling
    Authors: C Shen, A Nayak, LR Neitzel, AA Adams, M Silver-Ise, LM Sawyer, H Benchabane, H Wang, N Bunnag, B Li, DT Wynn, F Yang, M Garcia-Con, CH Williams, S Dakshanamu, CC Hong, NG Ayad, AJ Capobianco, Y Ahmed, E Lee, DJ Robbins
    Nature Communications, 2021-09-06;12(1):5263.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  4. CRL4AMBRA1 is a master regulator of D-type cyclins
    Authors: D Simoneschi, G Rona, N Zhou, YT Jeong, S Jiang, G Milletti, AA Arbini, A O'Sullivan, AA Wang, S Nithikasem, S Keegan, Y Siu, V Cianfanell, E Maiani, F Nazio, F Cecconi, F Boccalatte, D Fenyö, DR Jones, L Busino, M Pagano
    Nature, 2021-04-14;592(7856):789-793.
    Species: Human
    Sample Types: Peptide
    Applications: Bioassay
  5. Targeting a helix-in-groove interaction between E1 and E2 blocks ubiquitin transfer
    Authors: AM Cathcart, GH Bird, TE Wales, HD Herce, EP Harvey, ZJ Hauseman, CE Newman, U Adhikary, MS Prew, T Oo, S Lee, JR Engen, LD Walensky
    Nat. Chem. Biol., 2020-08-17;0(0):.
    Species: Human
    Sample Types: Cell Lysates, Protein
    Applications: Bioassay, Inhibition
  6. CD146 Regulates Growth Factor-Induced mTORC2 Activity Independent of the PI3K and mTORC1 Pathways
    Authors: W Xu, H Hua, YH Chiu, G Li, H Zhi, Z Yu, F Ren, Y Luo, W Cui
    Cell Rep, 2019-10-29;29(5):1311-1322.e5.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  7. Suppression of autophagy during mitosis via CUL4-RING ubiquitin ligases-mediated WIPI2 polyubiquitination and proteasomal degradation
    Authors: G Lu, J Yi, A Gubas, YT Wang, Y Wu, Y Ren, M Wu, Y Shi, C Ouyang, HWS Tan, T Wang, L Wang, ND Yang, S Deng, D Xia, RH Chen, SA Tooze, HM Shen
    Autophagy, 2019-03-30;0(0):1-18.
    Applications: Bioassay
  8. Peptide inhibitors of the anaphase promoting-complex that cause sensitivity to microtubule poison
    Authors: SC Schuyler, YO Wu, HY Chen, YS Ding, CJ Lin, YT Chu, TC Chen, L Liao, WW Tsai, A Huang, LI Wang, TW Liao, JH Jhuo, V Cheng
    PLoS ONE, 2018-06-08;13(6):e0198930.
    Applications: Bioassay
  9. Increased proteasomal activity supports photoreceptor survival in inherited retinal degeneration
    Authors: ES Lobanova, S Finkelstei, J Li, AM Travis, Y Hao, M Klingeborn, NP Skiba, RJ Deshaies, VY Arshavsky
    Nat Commun, 2018-04-30;9(1):1738.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  10. Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors
    Authors: TR de Moura, S Mozaffari-, CZK Szabó, J Schmitzová, O Dybkov, C Cretu, M Kachala, D Svergun, H Urlaub, R Lührmann, V Pena
    Mol. Cell, 2018-03-15;69(6):979-992.e6.
    Species: Bacteria
    Sample Types: Cell Lysates
    Applications: Bioassay
  11. Ubiquitinated proteins promote the association of proteasomes with the deubiquitinating enzyme Usp14 and the ubiquitin ligase Ube3c
    Authors: CL Kuo, AL Goldberg
    Proc. Natl. Acad. Sci. U.S.A., 2017-04-10;114(17):E3404-E3413.
    Applications: Bioassay
  12. Development of ?-Hairpin Peptides for the Measurement of SCF-Family E3 Ligase Activity in Vitro via Ornithine Ubiquitination
    Authors: KM Houston, AT Melvin, GS Woss, EL Fayer, ML Waters, NL Allbritton
    ACS Omega, 2017-03-29;2(3):1198-1206.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  13. Deltex2 represses MyoD expression and inhibits myogenic differentiation by acting as a negative regulator of Jmjd1c
    Authors: D Luo, A de Morree, S Boutet, N Quach, V Natu, A Rustagi, TA Rando
    Proc. Natl. Acad. Sci. U.S.A., 2017-03-28;114(15):E3071-E3080.
    Species: Mouse
    Sample Types: Protein
    Applications: Bioassay
  14. RNF11 sequestration of the E3 ligase SMURF2 on membranes antagonizes SMAD7 down-regulation of transforming growth factor ? signaling
    Authors: RJ Malonis, W Fu, MJ Jelcic, M Thompson, BS Canter, M Tsikitis, FJ Esteva, I Sánchez
    J. Biol. Chem., 2017-03-14;292(18):7435-7451.
    Applications: Bioassay
  15. UBE3B is a Calmodulin-Regulated, Mitochondria-Associated E3 Ubiquitin Ligase
    Authors: Andrea Braganza
    J. Biol. Chem, 2016-12-21;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  16. The Itch ubiquitin ligase is required for KSHV RTA induced vFLIP degradation
    Authors: Jennifer C Chmura
    Virology, 2016-11-30;501(0):119-126.
    Applications: Bioassay
  17. Porcine reproductive and respiratory syndrome virus Nsp1beta inhibits interferon-activated JAK/STAT signal transduction by inducing karyopherin-alpha1 degradation.
    Authors: Wang R, Nan Y, Yu Y, Zhang Y
    J Virol, 2013-02-28;87(9):5219-28.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay

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